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目的对一例疑似细菌性脑膜炎病例及密切接触者进行病原学检测。方法采集患者脑脊液、血液进行分离培养、乳胶凝集、Real-time PCR检测;密切接触者采集咽拭子标本32份进行分离培养、Real-time PCR检测。结果患者脑脊液经乳胶凝集、分离培养、Real-time PCR鉴定为W135群脑膜炎奈瑟菌引起的脑膜炎病例,密切接触者中患者父母分别检出W135群带菌。结论该病例为濮阳市首例W135群脑膜炎奈瑟菌病例,患者父母带菌与其患病密切相关,乳胶凝集法用于脑炎脑膜炎病例诊断及时方便,Real-time PCR法准确快速,在流脑带菌调查中能发挥重要作用。
Objective To examine the etiology of a case of suspected bacterial meningitis and close contacts. Methods Cerebrospinal fluid (CSF) and blood were collected for isolation and culture, latex agglutination and real-time PCR detection. Thirty-two throat swab specimens from close contacts were collected for isolation and culture and detected by real-time PCR. Results Cerebrospinal fluid (CSF) was agglutinated by latex, isolated and cultured. Real-time PCR was used to identify meningitis caused by Neisseria meningitidis in group W135. W135 group was detected in parents of close contacts. Conclusions The case was the first case of Neisseria meningitidis W135 in Puyang City. The incidence of parental infection was closely related to the disease. The latex agglutination method was used in the diagnosis of patients with encephalitis and meningitis in a timely and convenient manner. Real-time PCR was accurate and rapid. Brain infection investigation can play an important role.