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目的:研究恒山黄芪内生真菌Rhizopus sp.的次生代谢产物的类别。方法:通过组织学的方法从恒山黄芪中分离到AR02,真菌的鉴定采用形态学法结合分子生物学法,内生真菌大量发酵采用固体培养的方法,次生代谢产物的分离采用硅胶柱色谱法,Sephadex LH-20凝胶色谱法结合HPLC进行分离纯化,分离到的代谢产物的化学结构通过理化分析测定结合光谱分析进行鉴定。结果:从黄芪根部分离到的内生真菌AR02被鉴定为根霉属Rhizopus sp.,从该菌株的固体发酵产物中分离得到7个化合物,经结构鉴定,分别为色胺酮(1),lawsaritol A(2),曲菌酸(3),trypacidin(4),monomethylsulochrin(5),emodin 8-Omethylether(6)和cannabifolactone A(7)。结论:化合物1~3,6和7均为首次从恒山黄芪内生真菌中分离得到,且黄芪内生真菌Rhizopus sp.的次生代谢产物呈现出多个类别,可以作为活性物质的新来源。
Objective: To study the secondary metabolites of endophytic fungus Rhizopus sp. METHODS: AR02 was isolated from Astragalus membranaceus by histological method. The morphological and molecular biological methods were used to identify the fungi. The endophytic fungi were fermented in large amount by solid culture method. The secondary metabolites were isolated by silica gel column chromatography , Sephadex LH-20 gel chromatography combined with HPLC for separation and purification, the chemical structure of the isolated metabolites was identified by physicochemical analysis combined with spectral analysis. Results: The endophytic fungus AR02 isolated from Astragalus membranaceus was identified as Rhizopus sp. The seven compounds were isolated from the solid fermentation products of this strain and identified by structure, namely, tryptamine (1), lawsaritol A (2), Aspergillus (3), trypacidin (4), monomethylsulochrin (5), emodin 8-Omethylether (6) and cannabifolactone A (7). CONCLUSION: Compounds 1 ~ 3, 6 and 7 were isolated from endophytic fungi of Astragalus membranaceus for the first time, and the secondary metabolites of Rhizopus sp. Showed a variety of classes and could be used as new sources of active substances.