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本试验旨在研究不同来源木聚糖酶及其组合对木聚糖的水解效果,并研究不同木聚糖水解产物对细菌增殖及大肠杆菌对肠道上皮细胞黏附性的影响。试验用木聚糖酶A和木聚糖酶B分别来源于毕赤酵母和米曲霉。采用木聚糖酶A、木聚糖酶B、组合酶1(木聚糖酶A∶木聚糖酶B=3∶7)、组合酶2(木聚糖酶A∶木聚糖酶B=1∶1)、组合酶3(木聚糖酶A∶木聚糖酶B=7∶3)分别水解木聚糖,然后测定木聚糖水解产物对大肠杆菌、枯草芽孢杆菌和乳酸杆菌增殖和大肠杆菌对肠道上皮细胞黏附性的影响。结果表明:1)2种木聚糖酶有一定的组合效应,木聚糖酶A、木聚糖酶B、组合酶1、组合酶2、组合酶3组木二糖和木三糖的总含量分别为95.70%、86.79%、93.11%、94.55%和87.55%,其中木聚糖酶A组木二糖含量最高,组合酶2组木三糖含量最高。2)培养20 h时,5种木聚糖水解产物对大肠杆菌的增殖(以菌液吸光度值表示)没有产生显著影响(P>0.05);培养20和30 h时,5种木聚糖水解产物显著促进枯草芽孢杆菌的增殖(P<0.05);培养13和17 h时,5种木聚糖水解产物显著促进乳酸杆菌的增殖(P<0.05)。3)5种木聚糖水解产物均可以显著降低大肠杆菌对肠道上皮细胞的黏附率(P<0.05)。由此可见,通过不同来源木聚糖酶及其组合水解木聚糖,可以产生以木二糖和木三糖为主要组分的水解产物,从而起到促进枯草芽孢杆菌和乳酸杆菌增殖、减少大肠杆菌对肠道上皮细胞黏附的作用。
The aim of this study was to investigate the hydrolysis of xylan by different xylanases and their combinations and to study the effects of different xylan hydrolysates on bacterial proliferation and the adhesion of E. coli to intestinal epithelial cells. The experimental xylanase A and xylanase B were derived from Pichia and Aspergillus oryzae, respectively. Xylanase A, Xylanase B, Combinase 1 (xylanase A: xylanase B = 3: 7), Combinase 2 (xylanase A: xylanase B = 1: 1), combined enzyme 3 (xylanase A: xylanase B = 7: 3) to hydrolyze xylan, respectively, and then measuring the effect of the xylan hydrolyzate on the proliferation of Escherichia coli, Bacillus subtilis and Lactobacillus Effect of Escherichia coli on intestinal epithelial cell adhesion. The results showed that: 1) The combination of two xylanases had a certain combination effect. The total xylanase A, xylanase B, combined enzyme 1, combined enzyme 2, combined enzyme 3 groups of xylobiose and xylotriose The contents of xylanase A and xylanase A were the highest, and the content of xylo-saccharide was 95.70%, 86.79%, 93.11%, 94.55% and 87.55%, respectively. 2) When cultured for 20 h, the five xylan hydrolysates had no significant effect on the proliferation of Escherichia coli (indicated by the bacterial absorbance value) (P> 0.05). When cultured for 20 and 30 h, the five xylan hydrolyzates The product significantly promoted the proliferation of Bacillus subtilis (P <0.05). When cultured for 13 and 17 h, the five xylan hydrolysates significantly promoted the proliferation of Lactobacillus (P <0.05). 3) The 5 xylan hydrolyzate can significantly reduce the adhesion rate of Escherichia coli to intestinal epithelial cells (P <0.05). Thus, xylanase from different sources and their combination hydrolyzed xylan can produce xylobiose and xylotriose as the main component of hydrolysates, which can promote Bacillus subtilis and Lactobacillus proliferation, reduce Effect of Escherichia coli on intestinal epithelial cell adhesion.