论文部分内容阅读
目的探讨抗肿瘤药物羧胺三唑(CAI)是否具有抗炎镇痛作用并初步考察其作用机制。方法采用巴豆油诱导小鼠耳肿胀模型、棉球肉芽肿模型和大鼠佐剂性关节炎模型评价CAI的抗炎作用。检测CAI对血管内皮生长因子(VEGF)或组胺诱导的小鼠背部皮肤局部血管通透性的影响,以及对炎症部位和血清中致炎细胞因子肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)水平的影响。采用醋酸致小鼠扭体模型和福尔马林致痛模型评价CAI的镇痛作用。结果CAI对急慢性炎症均有明显抑制作用,对VEGF或组胺诱导的血管通透性增加有明显缓解作用,对致炎细胞因子TNF-α和IL-1β有明显抑制作用。CAI对外周炎性疼痛也有明显治疗作用。结论CAI是一种极具开发潜力的抗炎镇痛物质。
Objective To investigate whether anti-tumor drug carboxamide (CAI) has anti-inflammatory and analgesic effects and to investigate its mechanism. Methods The anti-inflammatory effect of CAI was evaluated by using croton oil-induced mouse ear swelling model, cotton ball granuloma model and rat adjuvant arthritis model. To detect the effect of CAI on the local vascular permeability of mouse skin induced by vascular endothelial growth factor (VEGF) or histamine, and the effect of CAI on the inflammation site and serum inflammatory cytokines such as tumor necrosis factor alpha (TNF-α) and leukocyte Effect of 1β (IL-1β) levels. The analgesic effect of CAI was evaluated by acetic acid-induced writhing model and formalin-induced pain model. Results CAI had obvious inhibitory effect on acute and chronic inflammation, relieved the increase of vascular permeability induced by VEGF or histamine, and significantly inhibited the production of inflammatory cytokines TNF-α and IL-1β. CAI also has a significant therapeutic effect on peripheral inflammatory pain. Conclusion CAI is a promising anti-inflammatory and analgesic substance.