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目的通过对煤工尘肺(CWP)患者血清中8项生物标记物水平测定及因子分析,为探讨CWP发生机制提供新的依据。方法采用酶联免疫吸附法检测116例CWP患者血清中IL-18、IL-18R、IL-8、s CD40、s CD40L、MCP-1、MMP-9及TIMP-9等8项指标水平,采用SPSS17.0软件对其进行因子分析。结果 CWP组与对照组比较,IL-18、IL-18R、IL-8、s CD40L和TIMP-9水平升高,差异均具有统计学意义(P<0.01),而s CD40、MCP-1和MMP-9水平则无明显改变(P>0.05)。8项原始指标信息量提取率最高者为93.3%,最低者为64.1%;经因子分析提取到4个公因子,分别为炎症因子(F1)、共刺激分子(F2)、基质金属蛋白酶及其组织抑制因子(F3)、细胞因子受体(F4),其方差贡献率分别为33.06%、21.60%、14.16%、13.43%,累计方差贡献率为82.25%。结论 CWP患者血清8项生物标记物相关因素依次为炎症因子、共刺激分子、基质金属蛋白酶与其组织抑制因子和细胞因子受体等4种,因子分析法可能对今后探讨CWP发病机制、治疗等起指导作用。
OBJECTIVE: To provide a new basis for exploring the pathogenesis of CWP through the determination of 8 biomarkers in sera of coal worker’s pneumoconiosis (CWP) patients and factor analysis. Methods Serum levels of IL-18, IL-18R, IL-8, sCD40, sCD40L, MCP-1, MMP-9 and TIMP-9 were detected by enzyme-linked immunosorbent assay in 116 patients with CWP. SPSS17.0 software for its factor analysis. Results The levels of IL-18, IL-18R, IL-8, CD40L and TIMP-9 in CWP group were significantly higher than those in control group (P <0.01) MMP-9 levels had no significant change (P> 0.05). The highest extraction rate of the eight indicators was 93.3%, while the lowest was 64.1%. Four common factors were extracted from factor analysis, namely inflammatory factor (F1), costimulatory molecule (F2), matrix metalloproteinase The contribution rates of tissue inhibitor (F3) and cytokine receptor (F4) were 33.06%, 21.60%, 14.16% and 13.43%, respectively. The cumulative variance contribution rate was 82.25%. Conclusions There are four kinds of inflammatory markers, costimulatory molecules, matrix metalloproteinases and their tissue inhibitors and cytokine receptors in CWP patients. The factor analysis method may be used to investigate the pathogenesis and treatment of CWP in the future Guidance.