实验证明一株经SV 40病毒转化的毛细血管扩张性共济失调症病人的皮肤成纤维细胞AT 5 BIVA,对电离辐射和博莱霉素高度敏感,对γ射线诱发的DNA合成抑制呈抗性。本文尝试用DNA介导基因转移法向AT细胞导入人基因组DNA或其酶切片段,未能获得稳定的对γ射线抗性的转化细胞。染色体核型分析证实AT 5 BIVA细胞的第11号染色体中的一条缺失或异常。本文对外源目的基因未能稳定植入AT细胞的原因进行了讨论。 The experiment demonstrated that a human dermal fibroblast AT 5 BIVA transformed with the SV 40 virus is highly sensitive to ionizing radiation and bleomycin, and is resistant to inhibition of γ-ray-induced DNA synthesis. In this article, attempts were made to introduce human genomic DNA or its restriction fragments into AT cells using a DNA-mediated gene transfer method, failing to obtain stable γ-ray-resistant transformed cells. Karyotype analysis confirmed a deletion or abnormality in chromosome 11 of the AT 5 BIVA cell. This article discusses the reasons for the failure to stably implant AT cells in foreign genes.