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鲎血变形细胞溶解物用于检测输液热原,已有报道,我院于1977年开始应用中国鲎(Tachypleus tri-dentatus)从事这方面的试验工作,现将情况简报如下。检测热原用试剂的制备和活性测定1.取血:无菌无热原条件下,在鲎胸甲与腹甲交接处中点穿刺取血,为使血液不致凝固,针管或盛血容器内放有与取血量等体积的抗凝剂。2.抗凝:N-乙基马来酰亚胺(NEM)、乙二胺四乙酸钠、咖啡因、黄嘌呤、硫酸铵对鲎血均有抗凝作用,但以0.125%:NEM 抗凝所制得的试剂活性较为稳定。
It has been reported in our hospital that Tachypleus tri-dentatus began to be used in this field in 1977, and the situation is as follows. Preparation of pyrogen test reagent and determination of activity 1. Blood: aseptic pyrogen free conditions, at the midpoint of the carapace and plastron puncture blood, in order to prevent the blood from coagulation, needle or blood vessel Placed with volume of blood and other anticoagulants. 2. Anticoagulation: N-ethylmaleimide (NEM), sodium ethylenediaminetetraacetate, caffeine, xanthine, ammonium sulfate have anticoagulant effects on blood stasis, but with 0.125%: NEM anticoagulation The resulting reagent activity is more stable.