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目的 探讨 1,2 5 - (OH) 2 D3 抑制肝癌细胞增殖的机制。方法 体外培养肝癌细胞株SMMC - 772 1,培养基添加 10、5 0及 10 0 (nmol/L) 1,2 5 - (OH) 2 D3 作用 2d、4d、6d后 ,用四唑盐比色试验 (MTT)和平板克隆形成实验检测细胞的存活和生长 ;台盼蓝拒染法绘制生长曲线 ;DNA凝胶电泳检测肝癌细胞凋亡。结果 MTT和平板克隆形成实验检测结果显示 10～ 10 0nmol/L的 1,2 5 - (OH) 2 D3 均对SMMC - 772 1细胞株有显著的抑制作用 ,且呈剂量 -效应关系。DNA凝胶电泳结果显示 1,2 5 - (OH) 2 D3 能够诱导SMMC - 772 1细胞凋亡。结论 1,2 5 - (OH) 2 D3 对于人肝癌细胞株SMMC - 772 1的增殖具有显著的抑制作用 ,其机理可能是通过干扰肝癌细胞的DNA代谢和诱导肝癌细胞凋亡。
Objective To investigate the mechanism of 1,2 5 - (OH) 2 D3 in inhibiting the proliferation of hepatoma cells. Methods Human hepatocellular carcinoma cell line SMMC - 772 1 was cultured in vitro. The cells were treated with 1,25 - (OH) 2 D3 at concentrations of 10, 50 and 10 0 (nmol / L) for 2, 4 and 6 days respectively. Cell viability and growth were assayed by MTT assay and plate clone formation assay. Growth curves were drawn by trypan blue exclusion method. Cell apoptosis was detected by DNA gel electrophoresis. Results The results of MTT assay and plate clone formation test showed that 10 ~ 100 nmol / L 1,2 5 - (OH) 2 D3 could significantly inhibit SMMC - 772 1 cell line with dose - effect relationship. DNA gel electrophoresis showed that 1,2 5 - (OH) 2 D3 could induce apoptosis of SMMC - 772 1 cells. Conclusions 1,25 - (OH) 2 D3 can significantly inhibit the proliferation of human hepatocellular carcinoma cell line SMMC - 772 1 by interfering with DNA metabolism and inducing apoptosis of hepatoma cells.