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目的:运用郭氏系统血液免疫反应分离实验体系,探讨在抗原激活的情况下异体白细胞、红细胞与自体白细胞、红细胞对反应体系中IL-8、IL-10影响的变化规律。方法:以人类大肠埃希菌作为激活原,加入异体白细胞、红细胞与自体白细胞、红细胞对比,37℃孵育1h,取上清液用ELISA测定IL-8、IL-10的分泌量。结果:IL-8的分泌量异体白细胞组(199.75970±62.743079)与自体白细胞组(465.92697±111.354713)相比明显下降(P=0.003<0.05),异体红细胞组(119.05455±31.056229)与自体红细胞组(132.63303±35.072837)相比无明显差异(P=0.456>0.05);IL-10的分泌量异体白细胞组(16.93582±2.346794)%与自体白细胞组相比(46.16061±9.180075)%明显下降(P=0.017<0.05),异体红细胞组(22.28497±4.010929)与自体红细胞组(27.58494±4.740832)相比无明显差异(P=0.274>0.05)。结论:同种异体同血型白细胞对自体血液免疫反应细胞因子的分泌具有负向的调控作用,同种异体同血型红细胞无此作用。
OBJECTIVE: To study the effects of leukocytes, leucocytes and autologous leucocytes and erythrocytes on the expression of IL-8 and IL-10 in the reaction system under the condition of antigen activation by using the system of Guo’s immune system. Methods: Human Escherichia coli was used as an activating progenitor. Allogeneic leucocytes, erythrocytes and autologous leucocytes were collected and incubated at 37 ℃ for 1 h. The supernatant was used to determine the secretion of IL-8 and IL-10. Results: Compared with autologous leukocyte group (465.92697 ± 111.354713), the secretion of IL-8 in allogeneic leukocyte group (199.75970 ± 62.743079) was significantly decreased (P = 0.003 <0.05), allogenic erythrocyte group (119.05455 ± 31.056229) 132.63303 ± 35.072837) (P = 0.456> 0.05). The secretion of IL-10 in leukocyte group (16.93582 ± 2.346794)% was significantly lower than that in autologous leukocyte group (46.16061 ± 9.180075)% <0.05). There was no significant difference (P = 0.274> 0.05) between allogeneic erythrocyte group (22.28497 ± 4.010929) and autologous erythrocyte group (27.58494 ± 4.740832). CONCLUSION: Allogeneic hematopoietic leukocytes have a negative regulatory effect on the secretion of autologous hemo-immune cytokines, and allogeneic hematopoietic cells have no such effect on erythrocytes.