论文部分内容阅读
目的:建立高效液相法同时测定红药片中三七皂苷R1和人参皂苷Rg1含量的方法。方法:液相条件Shim-PackVP-ODS柱,流动相为乙腈-0.05%磷酸溶液(20︰80),流速为1.0ml/min,检测波长为203nm,柱温为25℃。结果:三七皂苷R1的回归方程为Y=140994X+4793.2(r=0.9991,n=5),线性范围0.5255～1.5770μg,人参皂苷Rg1的回归方程为Y=128600X+178309(r=0.9993,n=5),线性范围2.103～6.308μg;其平均回收率分别为97.94%和98.91%。结论:此法简便、结果可靠,为红药片质量标准控制提供快速准确的测定方法。
Objective: To establish a HPLC method for the simultaneous determination of notoginsenoside R1 and ginsenoside Rg1 in red tablets. Method: The liquid phase condition was a Shim-Pack VP-ODS column. The mobile phase was acetonitrile-0.05% phosphoric acid solution (20..80), the flow rate was 1.0 ml/min, the detection wavelength was 203 nm, and the column temperature was 25°C. Results: The regression equation of notoginsenoside R1 was Y=140994X+4793.2(r=0.9991,n=5), the linear range was 0.5255～1.5770μg, and the regression equation of ginsenoside Rg1 was Y=128600X+178309(r=0.9993,n = 5), the linear range of 2.103 ~ 6.308μg; its average recovery was 97.94% and 98.91%, respectively. Conclusion: This method is simple, reliable, and provides a fast and accurate method for quality control of red pills.