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Chirality is an essential property of nature.The emergence of chiral material in nanoscale has shown great promise in the investigation of biological interfaces,chiral optical devices,metamaterials and the origin of chirality.In the present study,we focused on fabricating chiral nanoprobes and their applications in living systems.Through precise synthesis or the self-assembly of various chiral configurations,significant re-search achievements have been obtained by our group.In this brief introduction,we will discuss the re-cent progress of chiral nanostructures in the selective recognition of biomolecules,ultrasensitive detec-tion and their interaction with living cells.