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恶性肿瘤多药耐药现象与多种基因的表达有关。MDR_1在多种实体瘤及白血病中表达。多药耐药相关蛋白(MRP)是最近发现的另一种ATP依赖的药物排出泵。为探讨乳腺癌患者MDR_1及MRP表达的相互关系及其预后意义,作者应用RT-PCR及免疫组化法对134例乳腺癌患者进行研究。 手术切取肿瘤组织,立即—80℃存放,提取RNA,反转录为cDNA,以β_2微球蛋白为内参照进行PCR扩增,产物电泳、照相。免疫组化采用ABC法,分别选用针对MDR_1及MRP的单抗:Pgp及QCRL-1,QCRL-3。统计方法用卡方检验。结果RT-PCR检测MDR_1及MRP阳性率分别为60％(80/134)和100％(134/134)。
The phenomenon of multidrug resistance in malignant tumors is related to the expression of multiple genes. MDR_1 is expressed in various solid tumors and leukemias. Multidrug resistance-associated protein (MRP) is another recently discovered ATP-dependent drug excretion pump. To explore the relationship between MDR-1 and MRP expression in breast cancer patients and its prognostic significance, the authors applied RT-PCR and immunohistochemistry to study 134 breast cancer patients. The tumor tissue was surgically removed and stored at −80° C. immediately. RNA was extracted and reverse transcribed into cDNA. PCR amplification was performed using β 2 microglobulin as an internal reference. The product was electrophoresed and photographed. Immunohistochemistry using ABC method, respectively, for the selection of MDR_1 and MRP monoclonal antibodies: Pgp and QCRL-1, QCRL-3. The statistical method uses chi-square test. Results The positive rates of MDR-1 and MRP detected by RT-PCR were 60% (80/134) and 100% (134/134), respectively.