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目的:建立检测一种人血浆中丙戊酸钠液相色谱-质谱联用(LC-ESI-MS)的方法。方法:血浆中的药物用乙腈沉淀法去蛋白。采用Agilent Zorbax SB-C18(2.1 mm×100 mm,3.5μm)柱,流动相为乙腈-水(60:40,v/v)。采用电喷雾离子源(ESI),负离子检测,选择性离子监测(selected ion monitoring,SIM)方式定量分析丙戊酸钠,监测离子峰是m/z 143。结果:血浆中丙戊酸钠检测方法的线性范围为0.5μg/ml～100μg/ml,最低检测限为0.1μg/ml,平均回收率在89.7%～93.3%之间,日内和日间精密度RSD都小于8%。结论:本方法简单、灵敏、快速,可用于药物动力学研究。
Objective: To establish a method for the determination of sodium valproate in human plasma by liquid chromatography-mass spectrometry (LC-ESI-MS). Methods: The plasma drug was deproteinized by acetonitrile precipitation. An Agilent Zorbax SB-C18 (2.1 mm × 100 mm, 3.5 μm) column was used with mobile phase of acetonitrile-water (60:40, v / v). The sodium valproate was quantified by electrospray ionization (ESI), negative ion detection and selected ion monitoring (SIM), and the monitored ion peak was m / z 143. Results: The linear range of plasma sodium valproate was 0.5μg / ml ~ 100μg / ml, the lowest detection limit was 0.1μg / ml, the average recovery was between 89.7% ~ 93.3%. The intra- and inter-day precision RSD is less than 8%. Conclusion: The method is simple, sensitive and rapid and can be used for pharmacokinetic studies.