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本试验在产犊后立即无菌采集32头奶牛血液,其中17头为胎衣正常排出奶牛,15头为胎衣不下奶牛。胎衣正常排出奶牛在胎衣排出后立即采集胎盘,胎衣不下奶牛在确诊后再次静脉采血并采集其胎盘;用酶联免疫吸附测定法对胎衣不下奶牛和胎衣正常排出奶牛血清和胎盘中的激素含量进行检测,并对检测结果进行分析。结果表明,胎衣不下奶牛血清和胎盘中孕酮、纤维蛋白溶酶原和前列腺素(PGF_(2α))的含量与胎衣正常排出组相比差异显著降低(P<0.05),而雌二醇含量则显著升高(P<0.05)。本试验通过对胎衣不下和胎衣正常排出奶牛血清和胎盘中激素的含量进行检测并比较,结果发现奶牛胎衣不下的发生与血清和胎盘组织中的孕酮、雌二醇、纤维蛋白溶酶原和前列腺素(PGF_(2α))的含量变化有密切相关的联系,表明奶牛胎衣不下的发生可能与机体内激素分泌紊乱具有很大的关联。
The experiment immediately after calving aseptic collection of 32 cows blood, of which 17 were normal discharge of the cows, 15 cows no less than the cows. The normal pregnancy of the unwrapped cows collected the placenta immediately after the unwinding of the unwrapped cows, and the cows’ mothers drew the venous blood and collected the placenta again after the diagnosis. The content of corticosteroid in the cow serum and placenta Test, and analyze the test results. The results showed that the content of progesterone, plasminogen and prostaglandin (PGF_ (2α)) in serum and placenta were significantly lower than those in the normal pregnancy group (P <0.05), while the content of estradiol Was significantly higher (P <0.05). In the present study, the content of hormones in the serum and the placenta of the normal cowpeas and the placenta were detected and compared. The results showed that the occurrence of the unborn child in the cow was not related to the levels of progesterone, estradiol, plasminogen, Prostaglandin (PGF_ (2α)) content changes are closely related, indicating that the occurrence of dairy cows may have less than the body hormonal disorders have a great relationship.