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在高效液相色谱(HPLC)中,使用多柱串联能够极大地增加柱效和改善分离效果,提高温度能够明显降低色谱柱反压、加快分析速度。将色谱柱串联与提高柱温相结合构建了多柱串联高温HPLC系统,该系统常温下分析标准样品时(以芴计),绝对柱效达7.6×104塔板数;柱温升高到120℃,色谱柱反压从35.3MPa降低到15.4MPa,分析时间从78.39min缩短为19.26min。采用构建的系统分析中药六味地黄丸,常温下出峰94个,升高温度至100℃出峰80个,分离效果较常规的单柱系统有明显提高;升高温度,使主要组分的出峰时间缩短,可获得更为详尽的组成信息。
In high performance liquid chromatography (HPLC), the use of multiple columns in series can greatly increase the efficiency and improve the separation effect. Increasing the temperature can significantly reduce the column backpressure and speed up the analysis. A series of high temperature and multi-column HPLC systems were constructed by the combination of column chromatography and column temperature increase. When the standard sample was analyzed at room temperature (fluorene), the absolute column efficiency reached 7.6 × 104 plates. When the column temperature increased to 120 ℃, the column backpressure decreased from 35.3MPa to 15.4MPa, the analysis time was shortened from 78.39min to 19.26min. Using the constructed system to analyze the Chinese medicine Liuweidihuangwan, there are 94 peaks at room temperature, 80 peaks at 100 ℃, the separation effect is obviously improved compared with the conventional single-column system; the temperature is raised so that the main components Shorter peak time, get more detailed information.