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目的观察角质细胞生长因子(KGF)对胸腺上皮来源1C6细胞和4C18细胞的促增殖作用。方法鼠胸腺上皮髓质和皮质来源的1C6细胞、4C18细胞和鼠腹腔巨噬细胞来源的RAW细胞,分别经含不同浓度(25、50、100、200、400、800ng/ml)的KGF和不含KGF的完全培养液(对照组)作用24、48和72h,采用噻唑蓝(MTT)法测定细胞增殖率,并绘制细胞增殖率曲线。结果 MTT法检测显示,在低浓度(<200ng/ml)时,1C6细胞增殖率随KGF浓度增加不断升高;KGF浓度为200ng/ml时,1C6细胞增殖率达最高值;在高浓度(>200ng/ml)时,1C6细胞增殖率随KGF浓度的增加逐渐下降;与对照组比较,浓度为100、200、400ng/ml的KGF分别作用24、48和72h对1C6细胞均具有明显的促增殖作用(均P<0.05)。在低浓度(<100ng/ml)时,4C18细胞增殖率随KGF浓度增加不断升高;KGF浓度为100ng/ml时,4C18细胞增殖率达最高值;在高浓度(>100ng/ml)时,4C18细胞增殖率随KGF浓度的增加逐渐下降;与对照组比较,浓度为50、100、200、400ng/ml的KGF分别作用24、48和72h对4C18细胞均具有明显的促增殖作用(均P<0.05)。但不同浓度的KGF对RAW细胞均未见促增殖作用。结论 KGF对胸腺上皮来源的1C6细胞和4C18细胞均具有明显的促增殖作用。
Objective To observe the effect of keratinocyte growth factor (KGF) on the proliferation of 1C6 cells and 4C18 cells derived from thymus epithelium. Methods The primary cells of 1C6 cells, 4C18 cells and mouse peritoneal macrophage-derived RAW cells in the thymus and cortex of the thymus were treated with KGF at different concentrations (25, 50, 100, 200, 400 and 800 ng / ml) The complete culture medium containing KGF (control group) for 24, 48 and 72 hours, the cell proliferation rate was measured by MTT method and the cell proliferation rate curve was drawn. Results MTT assay showed that the proliferation rate of 1C6 cells increased with the increase of KGF concentration at low concentration (<200ng / ml); the proliferation rate of 1C6 cells reached the maximum at 200ng / ml KGF concentration; 200ng / ml), the proliferation rate of 1C6 cells decreased gradually with the increase of KGF concentration. Compared with the control group, KGF at the concentrations of 100, 200, and 400ng / ml for 24, 48 and 72h respectively had significant effects on the proliferation of 1C6 cells (All P <0.05). At low concentration (<100ng / ml), the proliferation rate of 4C18 cells increased with the increase of KGF concentration. When the concentration of KGF was 100ng / ml, the proliferation rate of 4C18 cells reached the highest value. At the high concentration (> 100ng / ml) The proliferation rate of 4C18 cells decreased gradually with the increase of KGF concentration. Compared with the control group, KGF at the concentrations of 50, 100, 200, and 400ng / ml for 24, 48 and 72h respectively had significant effects on the proliferation of 4C18 cells (P <0.05). However, different concentrations of KGF on RAW cells did not promote proliferation. Conclusion KGF can significantly promote the proliferation of 1C6 cells and 4C18 cells derived from the thymus epithelium.