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目的 研究光气急性吸入对机体抗氧化酶活力以及一氧化氮 (NO)、一氧化氮合酶(NOS)的影响。方法 利用三光气在N ,N 二甲基甲酰胺作用下分解生成光气的方法 ,设对照组采用SD大鼠进行动态恒量染毒 ,2h后处死实验动物 ,取肺组织测定湿干比 ,取全血、血清和肝组织分别测定谷胱甘肽硫转移酶 (GST)、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)、NO、NOS活力和总蛋白含量。结果 染毒组实验动物的肺湿干比明显大于对照组 ,差异有显著性(P <0 .0 1) ,血清和肝组织匀浆的GST活力明显升高 ,差异有显著性 (P <0 .0 5 ) ,血清和肝组织中SOD、全血CAT以及全血、血清、肝组织GSH Px活力明显提高 ,差异有显著性 (P <0 .0 5 ) ,血清和肝组织匀浆NO含量明显降低 ,差异有显著性 (P <0 .0 1) ,NOS活力明显升高 ,差异有显著性 (P <0 .0 1)。结论 大鼠光气急性吸入可以明显改变机体抗氧化酶系的活力 ,并且存在着一定程度的急性肝损伤 ,而这种损伤与活性氧密切相关 ,但多种抗氧化酶活力的升高却提示抗氧化治疗非光气急性中毒救治的首选。
Objective To study the effects of acute phosgene inhalation on antioxidant enzyme activity, nitric oxide (NO) and nitric oxide synthase (NOS) in the body. Methods The phosgene was decomposed by triphosgene under the action of N, N-dimethyl formamide. The rats in the control group were exposed to the constant dose of SD rats. After 2 hours, the animals were sacrificed and the lungs were taken for determination of wet-dry ratio The levels of glutathione S-transferase (GST), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH Px) NO, NOS activity and total protein content. Results The lung wet-dry ratio of the experimental animals was significantly higher than that of the control group (P <0.01). The serum and liver homogenate GST activity was significantly increased, the difference was significant (P <0 (P <0.05). The content of NO in serum and liver homogenate of SOD, whole blood CAT and whole blood, serum and liver tissue GSH Px increased significantly (P <0.05) (P <0.01). The activity of NOS was significantly increased, the difference was significant (P <0.01). Conclusion Acute inhalation of phosgene in rats can significantly change the activity of antioxidant enzymes in the body, and there is a certain degree of acute liver injury, which is closely related to reactive oxygen species, but the increase of the activity of many antioxidant enzymes suggests Antioxidant treatment of non-phosgene acute poisoning treatment of choice.