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目的：观察虎杖甙（ＰＤ）对培养的大鼠血管平滑肌细胞（ＶＳＭＣ）内钙离子浓度的变化机理。方法：用Ｆｌｕｏ－３－ＡＭ标记培养的ＶＳＭＣ，在粘附式细胞仪上测定细胞内游离钙的变化。结果：实验结果表明，ＰＤ（００２～２０ｍｍｏｌ／Ｌ）可使大鼠ＶＳＭＣ内游离钙浓度升高，波形变宽，其引起的钙波形态与去甲肾上腺素（ＮＥ）及氯化钾所引起的高尖钙波不同，ＰＤ使ＶＳＭＣ产生一种剂量依赖性的、持续缓慢升高的钙波。ＰＤ引起的细胞内游离钙升高可被ＥＧＴＡ（２ｍｍｏｌ／Ｌ）及维拉帕米（５０μｍｏｌ／Ｌ）明显抑制。结论：以上结果提示ＰＤ既促进ＶＳＭＣ外钙离子进入细胞内，还能诱导细胞内钙离子释放；ＰＤ可能会提高正常ＶＳＭＣ的收缩性，增加正常血管的张力。
Objective: To observe the change mechanism of calcium ion concentration in cultured rat vascular smooth muscle cells (VSMC) with Polygonum cuspidatum (PD). Methods: Cultured VSMCs were labeled with Fluo-3-AM and intracellular free calcium was measured on an adherent cytometer. Results: The experimental results showed that PD (002～20mmol/L) could increase the free calcium concentration in rat VSMC, and the waveform became wider, which caused the calcium wave morphology and norepinephrine (NE) and chlorine. Different from the high-grade calcium wave caused by potassium, PD causes VSMC to produce a dose-dependent, sustained and slowly rising calcium wave. The increase of intracellular free calcium induced by PD was significantly inhibited by EGTA (2 mmol/L) and verapamil (50 μmol/L). Conclusion: The above results suggest that PD not only promotes extracellular calcium ion entry into the cell, but also induces intracellular calcium release; PD may increase the contractility of normal VSMCs and increase the tension of normal vessels.