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目的研究我国2型糖尿病(T2DM)人群脂联素受体1(AdipoR1)基因多态性与冠心病(CAD)风险的相关性。方法以307例T2DM患者为研究人群,其中205例伴有CAD,102例不伴CAD。应用聚合酶链式反应-限制性内切酶片断长度多态性(PCR-RFLP)技术或基因测序方法 ,研究AdipoR1 10个单倍型标记单核苷酸多态性(Haplotype-tagging SNPs)与CAD风险的关系。结果 (1)AdipoR1SNP rs1342387 GG和AG基因型携带者均较AA型携带者发生CAD的风险显著增加(GG vsAA:校正OR′=2.491,95%CI′1.354～6.885,P′=0.031;AG vs AA:校正OR′=3.053,95%CI′1.085～5.718,P′=0.007)。(2)SNP rs12045862基因型CC携带者与TT携带者相比,CAD的风险显著增加(校正OR′=2.751,95%CI′1.063～7.115,P′=0.037)。(3)SNPs rs1342387、rs12045862 GX/CC基因型组合携带者较非携带者CAD的风险显著增加(校正OR′=3.646,95%CI′1.253～10.608,P′=0.018)。保护性基因型组合AA/TX携带者较非携带者发生CAD的风险显著降低(校正OR′=0.260,95%CI′0.113～0.601,P′=0.002)。(4)在超重和肥胖(BMI≥24)的T2DM人群中,发现肥胖与SNPs rs1342387、rs12045862对CAD的风险有相互作用(P<0.05);在总人群中发现的与CAD风险相关的SNPs rs1342387和rs12045862在这一亚组仍具有相关性;同时发现SNP rs7539542基因型CC携带者较GG携带者CAD的风险增加(g正OR′=4.714,P′=0.036)。结论中国T2DM人群中,AdipoR1 SNPs与CAD的风险可能相关。在超重或肥胖T2DM人群中,AdipoR1 SNPs与肥胖对CAD风险的影响有协同作用。
Objective To investigate the association between AdipoR1 gene polymorphism and risk of coronary heart disease (CAD) in type 2 diabetes mellitus (T2DM) in China. Methods A total of 307 patients with T2DM were enrolled in this study. Among them, 205 patients had CAD and 102 patients had no CAD. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique or gene sequencing method was used to study the association of 10 haplotype SNPs in AdipoR1 with CAD risk relationship. Results (1) AdipoR1 SNP rs1342387 The risk of CAD in GG and AG carriers was significantly higher than that in AA carriers (GG vs AA: corrected OR = 2.491, 95% CI: 1.354-6.885, P ’= 0.031; AG vs AA: corrected OR ’= 3.053, 95% CI’ 1.085-5.718, P ’= 0.007). (2) The risk of CAD was significantly increased in SNP rs12045862 genotype CC carriers compared with TT carriers (adjusted OR = 2.751, 95% CI ’1.063-7.115, P’ = 0.037). (3) The SNPs rs1342387, rs12045862 GX / CC genotype carriers significantly increased the risk of CAD compared with non-carriers (adjusted OR ’= 3.646,95% CI’1.253 ~ 10.608, P’ = 0.018). The risk of developing CAD with protective genotypes AA / TX carriers was significantly lower than that of non-carriers (adjusted OR ’= 0.260, 95% CI’0.113-0.601, P’ = 0.002). (4) In T2DM population with overweight and obesity (BMI≥24), the association between obesity and SNPs rs1342387 and rs12045862 was found to be associated with the risk of CAD (P <0.05). The SNPs rs1342387 And rs12045862 still have correlation in this subgroup. At the same time, we found that the risk of CC carriers of SNP rs7539542 genotype was higher than that of GG carriers (g positive OR ’= 4.714, P’ = 0.036). Conclusions AdipoR1 SNPs may be associated with the risk of CAD in Chinese T2DM population. In overweight or obese T2DM populations, AdipoR1 SNPs have a synergistic effect on the risk of CAD with obesity.