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目的:探讨荧光原位杂交(FISH)技术在复发性流产患者病因分析中的应用价值。方法:根据孕妇年龄将116例复发性流产患者分为高龄组(≥35岁)30例和非高龄组(<35岁)86例,应用FISH技术对其流产绒毛组织的13、16、18、21、22、X和Y染色体非整倍体进行快速检测。结果:检测成功率为100%,共测出染色体异常53例,异常率为45.69%,其中常染色体三体29例,性染色体数目异常11例,性染色体单体合并常染色体三体1例,三倍体7例,四倍体5例。高龄组孕妇中绒毛染色体异常的检出率与非高龄组比较差异无统计学意义(P>0.05)。结论:胚胎染色体异常是复发性流产的重要诱发因素,应用FISH技术能快速、简便地检测出绒毛细胞的染色体数目异常情况,具有一定的临床应用价值。
Objective: To investigate the value of fluorescence in situ hybridization (FISH) in the etiological analysis of patients with recurrent spontaneous abortion. Methods: According to the age of pregnant women, 116 patients with recurrent spontaneous abortion were divided into 30 cases in the advanced age group (≥35 years old) and 86 cases in the non-advanced age group (<35 years old). FISH was used to detect the 13, 21, 22, X and Y chromosome aneuploidy for rapid detection. Results: The detection success rate was 100%, a total of 53 cases of chromosomal abnormalities were detected, the abnormal rate was 45.69%, including 29 cases of autosomal trisomy, 11 cases of abnormal sex chromosome number, 1 sex chromosome combined with autosomal trisomy, Triploid in 7 cases, tetraploid in 5 cases. There was no significant difference in the detection rate of chromosomal abnormalities among the pregnant women in the advanced age group compared with the non-advanced age group (P> 0.05). Conclusion: Embryo chromosomal abnormalities is an important inducing factor of recurrent spontaneous abortion. FISH can detect the abnormal chromosome number of villus cells quickly and easily, and has certain clinical value.