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针对在广西横县发生的一种王草叶斑病害,为明确其病原种类,采用形态学和分子生物学方法进行了鉴定,并研究其生物学特性。结合致病菌的形态特征以及ITS序列系统聚类分析结果,将其鉴定为草茎点霉(Phoma herbarum)。生物学特性研究表明:该菌菌丝体适宜生长的温度为20~32℃,最适温度为25~28℃;菌丝体生长的最适p H值为5~8;菌丝体生长的最佳碳源为葡萄糖、蔗糖和甘露醇;最佳氮源为蛋白胨,而尿素则不适合菌丝体生长;光/暗交替或暗/光交替均有利于菌丝体生长;菌丝体致死温度为50℃,10 min。
In order to clarify the pathogen species of a king leaf grass spot disease in Hengxian, Guangxi, morphological and molecular biological methods were used to identify and study its biological characteristics. The pathogen was identified as Phoma herbarum by combining with the morphological characteristics of pathogenic bacteria and cluster analysis of ITS sequences. The biological characteristics of the mycelium showed that the suitable growth temperature was 20 ~ 32 ℃, the optimum temperature was 25 ~ 28 ℃, the optimum p H value of mycelial growth was 5 ~ 8, mycelium growth The best carbon source is glucose, sucrose and mannitol. The best nitrogen source is peptone, while urea is not suitable for mycelium growth. Light / dark alternation or dark / light alternation are conducive to the growth of mycelia; mycelium death Temperature is 50 ℃, 10 min.