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本文采用离子交换层析和羟基磷灰石吸附层析技术从螺旋藻中提取纯化得到藻蓝蛋白和别藻蓝蛋白。通过比较 ＡＮＸ Ｓｅｐｈａｒｏｓｅ ４ Ｆａｓｔ Ｆｌｏｗ（ｈｉｇｈ ｓｕｂ／ｌｏｗ ｓｕｂ）、 ＤＥＡＥ Ｓｅｐｈａｒｏｓｅ Ｆａｓｔ Ｆｌｏｗ和 Ｑ Ｓａｐｈａｒｏｓｅ Ｆａｓｔ Ｆｌｏｗ等阴离子交换树脂的动态吸附容量以及目标产品的纯度，选用 ＤＥＡＥＳｅｐｈａｒｏｓｅ Ｆａｓｔ Ｆｌｏｗ作为层析介质．对离子交换的产物进行了电泳分析，藻蓝蛋白和别藻蓝蛋白的等电点接近，电迁移速率相似。采用羟基磷灰石吸附技术对藻胆蛋白混合物进一步分离纯化，分别得到了藻蓝蛋白和别藻蓝蛋白的纯品，经等电聚焦实验验证显示为均一组成。
In this paper, ion exchange chromatography and hydroxyapatite adsorption chromatography technology was extracted from Spirulina phycocyanin and allophycocyanin. DEA Sepharose Fast Flow was used as a chromatographic medium by comparing the dynamic adsorption capacity of anion exchange resins such as ANX Sepharose 4 Fast Flow (high sub / low sub), DEAE Sepharose Fast Flow and Q Sapharose Fast Flow, and the purity of the target product. The products of ion exchange were analyzed by electrophoresis. The isoelectric points of phycocyanin and allophycocyanin were similar and their electromigration rates were similar. Hydroxyapatite adsorption technique was used to separate and purify the phycobiliprotein mixture. Pure phycocyanin and allophycocyanin were obtained respectively. The isoelectric focusing experiment showed that the phycobiliproteins were homogeneous.