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目的建立钩藤复方制剂中钩藤碱、异钩藤碱、去氢钩藤碱和异去氢钩藤碱HPLC定量方法。方法 Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-乙腈-醋酸铵缓冲液(pH=5.2)梯度洗脱,流速:1 mL.min-1,检测波长:245 nm,柱温45℃。结果 4种生物碱线性分别为:钩藤碱(rhynchophylline,RIN):1.76~26.40 mg.L-1,异钩藤碱(isorhynchophylline,IRN):1.66~24.90 mg.L-1,去氢钩藤碱(corynoxeine,COR):2.50~37.50 mg.L-1,异去氢钩藤碱(isocorynox-eine,ICO):1.64~24.60 mg.L-1,r均大于0.999 7,平均回收率分别为98.3%~103.4%,RSD<2.5%。采用上述HPLC测定了天麻钩藤饮模型制剂和3种市售钩藤复方制剂中4种钩藤生物碱成分的含量。结论本法可作为复方制剂中4种钩藤生物碱成分的含量测定。
OBJECTIVE To establish a HPLC method for the determination of rhynchophylline, isorhamnetine, rhynchophylline and isorhamnetin in Uncaria compound. Methods The mobile phase was eluted with a gradient of methanol-acetonitrile-ammonium acetate buffer (pH = 5.2) on a Kromasil C18 column (250 mm × 4.6 mm, 5 μm) at a flow rate of 1 mL · min- Column temperature 45 ℃. Results Linearity of the four alkaloids were as follows: rhynchophylline (RIN): 1.76-26.40 mg.L-1, isorhynchophylline (IRN): 1.66-24.90 mg.L-1, The average recovery of corynoxeine (COR): 2.50 ~ 37.50 mg.L-1 and isocorynox-eine (ICO): 1.64 ~ 24.60 mg.L- 98.3% ~ 103.4%, RSD <2.5%. The contents of four kinds of Uncaria alkaloids in Tianma gudgeon model and three kinds of Uncaria rhynchophylla compound prescriptions were determined by HPLC. Conclusion This method can be used as a compound preparation of four kinds of Uncaria alkaloids content determination.