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本文研究了注射不同剂量的小肽对刺参体腔液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和超氧化物歧化酶(SOD)活性及抗病力的影响。各试验组(0.2 mg组、0.4 mg组、0.6 mg组)分别注射浓度为0.4、0.8、1.2 mg/mL的小肽溶液500μL/只,对照组注射等体积无菌过滤海水。在注射后第2、4和7天,检测刺参体内ACP、AKP和SOD活性。结果表明,试验组刺参体腔液中ACP(0.4 mg组)、AKP(0.4 mg组)活性及SOD(0.6 mg组)活性分别在第4、4、7天达到最高值,与对照组相比差异均显著(P<0.05),分别为对照组的2.6、2.3、1.9倍。攻毒试验结果证实,各试验组均产生了一定的免疫保护率,0.4 mg组最大,达33.3%。结果提示,小肽作为饲料添加剂能够提高刺参的非特异性免疫水平,且注射量为0.4 mg效果最好。
In this paper, the effects of different doses of small peptides on the activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and superoxide dismutase (SOD) and their resistance to disease in vivo were studied. Each test group (0.2 mg group, 0.4 mg group, 0.6 mg group) was injected with 500 μL of small peptide solution of 0.4, 0.8, 1.2 mg / mL respectively, and the control group was injected with equal volume of sterile filtered seawater. The ACP, AKP and SOD activities in Apostichopus japonicus were detected on the 2nd, 4th, and 7th day after injection. The results showed that the activities of ACP (0.4 mg group), AKP (0.4 mg group) and SOD (0.6 mg group) in the body fluid of Apostichopus japonicus group reached the highest values on the 4th, 4th, and 7th days respectively. Compared with the control group The difference was significant (P <0.05), respectively, 2.6, 2.3, 1.9 times the control group. Toxicity test results confirmed that each experimental group had a certain degree of immune protection, 0.4 mg group was the largest, up to 33.3%. The results suggest that small peptides as a feed additive can improve the nonspecific immune level of sea cucumber, and injection volume of 0.4 mg is the best.