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Cohen等(1982)在《寄生虫感染的免疫》一书中强调指出:“没有精细的抗原和抗体的分析,对宿主-寄生虫关系的免疫学研究不可能有进展”。抗原分析是免疫寄生虫学发展的客观要求,与一定的技术和方法相联系。为叙述方便,现分物理与化学,免疫学和生物学方法作一综述, 一、物理与化学方法(一) 化学方法1.化学试剂直接分析法:据横川等(1958)报道,用卫氏并殖吸虫成虫的酚不溶性和酚溶性抗原对并殖吸虫流行区居民进行皮试,发现具有皮试抗原活性的物质存在于酚溶性物质(蛋白)中,而不存在于酚不溶
Cohen et al. (1982) emphasized in his book “Immunity in Parasitic Infections,” that “there is no way that immunological research on host-host relationships can progress without the analysis of fine antigens and antibodies.” Antigen analysis is an objective requirement for the development of immuno-parasitology and is linked to certain techniques and methods. For the convenience of the narrative, is divided into physical and chemical, immunological and biological methods are reviewed, a physical and chemical methods (A) chemical methods 1. Direct chemical reagent analysis: According to Yokogawa et al. (1958) Paracarpus phenols insoluble and phenol-soluble antigens Paragonimus inhabitants endemic skin test and found that skin test antigen activity of substances present in the phenol-soluble substances (proteins), but not in the phenol-insoluble