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目的:建立穿心莲抗炎片中穿心莲内酯和脱水穿心莲内酯含量测定方法。方法:采用高效液相色谱法测定,色谱柱为AgiLent SB C_(18)柱(4.6mm×250mm,5μm),流动相为甲醇-水(58:42),检测波长为225 nn,柱温为30℃,理论塔板数按穿心莲内酯和脱水穿心莲内酯峰面积计算应不低于3 000。结果:穿心莲内酯在0.168～1.680μg,脱水穿心莲内酯在0.409～4.090μg范围内线形关系良好,r=0.9999。穿心莲内酯和脱水穿心莲内酯平均回收率为99.07%和98.91%,RSD=0.75%和0.79%。结论:该方法准确性、重复性好,可用于穿心莲抗炎片的质量标准控制。
Objective: To establish a method for determination of andrographolide and dehydroandrographolide in Tongxinlian anti-inflammatory tablets. Methods: The HPLC method was used for the determination of AgiLent SB C 18 column (4.6mm×250mm, 5μm). The mobile phase was methanol-water (58:42). The detection wavelength was 225 nn. The column temperature was At 30 °C, the number of theoretical plates should not be less than 3 000 calculated on the area of the andrographolide and dehydroandrographolide peaks. RESULTS: Andrographolide ranged from 0.168 to 1.680 μg, and dehydroandrographolide had good linearity in the range of 0.409 to 4.090 μg, r=0.9999. The average recoveries of andrographolide and dehydroandrographolide were 99.07% and 98.91%, and RSDs were 0.75% and 0.79%, respectively. Conclusion: The method is accurate and repeatable, and can be used to control the quality standard of anti-inflammatory tablets of Andrographis paniculata.