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玉米自交系幼胚在添加 1～ 2mg/L 2 ,4-D的N6培养基上能够诱导形成不同类型的胚性愈伤组织。愈伤组织的诱导率与基因型和 2 ,4-D浓度有关 ,6个自交系的诱导率在 5 2 %～ 89%。将淡黄色颗粒状结构致密型愈伤组织在N6诱导培养基上继代培养 2 0个月 ,仍具有较强的分化能力。在附加 2mg/L 6 -BA和0 .2mg/LNAA的N6分化培养基上 ,有 2 7%～ 41%的愈伤组织分化成苗。通过基因枪轰击成功地将 pbarGUS基因转入玉米细胞 ,获得了大量的转化胚性愈伤组织。用 0 .4mol/L的甘露醇高渗处理幼胚 ,能显著提高pbarGUS基因在细胞中的表达 ,高渗处理转化率较对照提高了 3倍
The immature embryos of maize inbred lines could induce different types of embryogenic callus on N6 medium supplemented with 1 ~ 2mg / L 2, 4-D. The induction rate of callus was related to the genotype and concentration of 2,4-D, and the inducibility of 6 inbred lines ranged from 52% to 89%. The light yellow granular dense callus on the N6 induction medium subculture 20 months, still has strong differentiation ability. On N6 differentiation medium supplemented with 2 mg / L 6-BA and 0.2 mg / L NAA, 27 to 41% callus differentiated into seedlings. Transgenic pbarGUS gene was successfully transferred into maize cells by gene gun bombardment, and a large number of transformed embryogenic callus were obtained. Immature embryos treated with 0.4 mol / L mannitol significantly increased the expression of pbarGUS gene in the cells, and the conversion rate of hypertonic treatment was increased by 3 times compared with the control