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目的 研究人骨髓瘤细胞系U2 6 6中的白细胞介素 6 (IL 6 )信号转导途径及彼此之间的相互调控方式。方法 首先采用凝胶阻滞电泳 (electrophoreticmobilityshiftassay,EMSA)方法观察分别参与两条IL 6信号转导途径的转录因子STAT3和NF IL 6在U2 6 6细胞中的诱导激活状态并确定该细胞中的IL 6信号转导通路 ;继而采用转基因或化学试剂处理方法 ,特异性上调或下调其中一条IL 6信号途径的活化 ,并同时观察另外一条信号途径的激活状态。结果 1.以上两种转录因子分别参与的IL 6信号转导途径———JAK/STAT和Ras/NF IL 6 ,它们都能够在U2 6 6细胞中诱导激活 ;2 .在高剂量IL 6 (1～ 10 0ng/ml)刺激范围之内 ,一条IL 6信号途径活化水平的升高可同时导致另一条信号途径活化水平的下降。结论 在一定IL 6刺激剂量范围内 ,U2 6 6细胞中两条IL 6信号途径的诱导激活存在着相互拮抗作用。
Objective To investigate the interleukin 6 (IL 6) signal transduction pathways and their mutual regulation between human myeloma cell line U266. Methods Electrophoretic mobility shift assay (EMSA) was used to observe the activation of STAT3 and NF IL 6, two transcription factors involved in the IL 6 signaling pathway, in U 266 cells and to determine IL in the cells. 6 signal transduction pathway; followed by the use of transgenic or chemical reagent treatment, specifically up or down the activation of one of the IL 6 signaling pathway, and observe the activation of another signal pathway. Results 1. IL 6 signaling pathways involved in the above two transcription factors, JAK/STAT and Ras/NF IL 6, respectively, were able to induce activation in U266 cells; 2. In high-dose IL 6 ( Within the stimulation range of 1 to 10 0 ng/ml, an increase in the activation of an IL 6 signaling pathway may simultaneously lead to a decrease in the activation level of the other signaling pathway. Conclusion There is an antagonistic effect on the activation of two IL 6 signaling pathways in U2 6 6 cells within a certain IL 6 stimulation dose range.