启动子是细胞内控制基因转录与表达的时空特异性的重要因子。玉米醇溶蛋白基因Zein启动子控制Zein基因在胚乳中特异性表达,分离Zein启动子将为外源基因在胚乳中特异性表达等遗传操作提供基础。以玉米自交系鲁2548基因组DNA为模版,PCR扩增得到401 bp的15 kuβ-Zein启动子,PCR产物T-A克隆并测序。序列分析结果显示,作者克隆的Zein启动子序列与NCBI已报道的15 ku-Zein基因(GenBank M72708)启动子序列同源性为96%。启动子功能预测及顺式元件分析表明,所克隆的启动子序列可能具有胚乳特异性启动子功能。有关功能验证试验正在开展之中。本研究为外源基因胚乳特异性表达基因工程奠定了基础。 Promoters are important factors controlling the temporal and spatial specificity of intracellular transcription and expression of genes. The Zein promoter of Zein controls the specific expression of Zein gene in endosperm, and the isolation of the Zein promoter will provide the basis for genetic manipulation of exogenous genes in the endosperm. A 401 bp 15 kuβ-Zein promoter was amplified by PCR using the maize inbred line Lu 2548 genomic DNA. The PCR product was cloned and sequenced. Sequence analysis showed that the cloned Zein promoter sequence was 96% homologous to the 15 ku-Zein gene (GenBank M72708) reported by NCBI. Promoter function prediction and cis-element analysis showed that the cloned promoter sequence may have endosperm-specific promoter function. Functional verification tests are underway. This study laid the foundation for gene engineering of endosperm-specific expression of exogenous gene.