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目的 :分析结肠癌相关转录因子1(CCAT-1)m RNA在结直肠癌患者外周血中的表达水平与临床病理特征及预后生存的关联,探讨CCAT-1在结直肠癌早期筛查及病程预后评估中的作用。方法:采用实时定量PCR法(q PCR)检测结直肠癌(60例)、结直肠腺瘤(30例)及健康志愿者(30例)外周血中CCAT-1 m RNA表达,采用ROC曲线判断CCAT-1对结直肠癌的诊断价值,计算Youden指数确定CCAT-1对结直肠癌的诊断阈值;采用双变量相关(bivariate correlation)Pearson检验分析CCAT-1m RNA表达水平与结直肠癌患者临床病理特征;采用Kaplan-Meier生存分析(Log-rank检验)分析CCAT-1 m RNA表达与结直肠癌患者3年生存率的相关性,绘制生存曲线。结果:结直肠癌患者外周血CCAT-1 m RNA水平相对于结直肠腺瘤患者(P<0.01)、健康志愿者(P<0.01)显著高表达,CCAT-1 m RNA在健康志愿者、结直肠腺瘤及结直肠癌患者外周血中的表达量呈逐渐递增趋势;结直肠癌患者外周血CCAT-1 m RNA表达量与肿瘤直径(P<0.05)、分化程度(P<0.01)、原发灶浸润深度(P<0.01)、淋巴结转移数(P<0.01)、远处转移(P<0.05)及TNM分期(P<0.05)呈显著正相关,与3年生存率呈负相关(P<0.05);CCAT-1 m RNA高表达组生存时间显著低于低表达组(P<0.01)。结论:CCAT-1可作为一种有价值的结直肠癌早期筛查及预后评估的标志物。
OBJECTIVE: To analyze the correlation between the expression of CCAT-1 m RNA in peripheral blood of patients with colorectal cancer and the clinicopathological features and prognosis of CCT-1, The role of prognostic assessment. Methods: The expression of CCAT-1 mRNA in peripheral blood of colorectal cancer (60 cases), colorectal adenoma (30 cases) and healthy volunteers (30 cases) was detected by real-time qPCR method. The diagnostic value of CCAT-1 for colorectal cancer was calculated, and the Youden index was calculated to determine the diagnostic threshold of CCAT-1 for colorectal cancer. The bivariate correlation Pearson test was used to analyze the expression of CCAT-1mRNA in colorectal cancer patients The Kaplan-Meier survival analysis (Log-rank test) was used to analyze the correlation between the expression of CCAT-1 mRNA and the 3-year survival rate of patients with colorectal cancer, and the survival curves were drawn. Results: The level of CCAT-1 m RNA in peripheral blood of patients with colorectal cancer was significantly higher than that of patients with colorectal adenoma (P <0.01), and was significantly higher in healthy volunteers (P <0.01). CCAT-1 mRNA was found in healthy volunteers The expression of CCAT-1 m RNA in peripheral blood of patients with colorectal cancer was significantly correlated with tumor diameter (P <0.05), differentiation degree (P <0.01), original (P <0.01), lymph node metastasis (P <0.01), distant metastasis (P <0.05) and TNM stage (P <0.05), but negatively correlated with 3-year survival rate <0.05). The survival time of CCAT-1 m RNA overexpression group was significantly lower than that of low expression group (P <0.01). Conclusions: CCAT-1 is a valuable marker for early screening and prognostic assessment of colorectal cancer.