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本实验利用Solt-Farber顺序诱发大鼠肝癌,观察肝组织中GST活性及GST-P含量在化学诱癌中的变化,并观察性激素对大鼠化学诱发肝癌的早期病变中GST-P表达的作用。结果显示无论是GST活性或GST-P的含量,在诱癌至第三周开始升高,第五周升至最高。利用此模式,选择诱癌至第五周,免疫组化法检测各种处理后肝组织中GST-P的表达。发现睾丸假切除的雄性大鼠经化学诱癌后,肝中有高的GST-P表达,睾丸假切除的雄性大鼠诱癌合并雌二醇处理,明显降低肝组织GST-P阳性灶的面积和数量;合并睾丸酮处理,虽减少GST-P阳性灶的面积,但其数量略有升高。与睾丸假切除后诱癌的雄性大鼠相比,切除睾丸的大鼠经诱癌,有更低的GST-P阳性灶的面积;睾丸切除合并雌二醇处理,GST-P阳性灶的面积进一步降低。与仅化学诱癌的卵巢假切除雌性大鼠比,卵巢切除鼠诱癌后,GST-P阳性灶的面积稍有增加;对卵巢切除合用睾丸酮的大鼠诱癌,阳性灶的面积进一部增加。无论性腺切除与否,雄性大鼠比雌性大鼠有更高的GST-P表达。这些结果提示雌激素可抑制而雄激素则可促进化学诱癌大鼠肝中GST-P的表达。这一结果可能与临床上男性较女性易患肝癌有关。
In this experiment, Solt-Farber sequence was used to induce rat liver cancer, and the changes of GST activity and GST-P content in chemical liver cancer were observed, and the effect of sex hormone on GST-P expression in early lesions of chemically induced liver cancer in rats was observed. . The results showed that whether the GST activity or GST-P content increased from the third week to the third week, the fifth week rose to the highest. Using this model, the induction of cancer was selected until the fifth week. Immunohistochemistry was used to detect the expression of GST-P in various liver tissues after treatment. Male rats with testicular excision were found to have high GST-P expression in the liver after chemical induction of cancer, and male rats with sham excision test were treated with estradiol to significantly reduce the area of GST-P positive foci in liver tissue. And the number; merger of testosterone treatment, although reducing the area of GST-P positive lesions, but its number increased slightly. Rats with excised testes had an area of GST-P-positive foci compared to male rats induced by testicular excision; testes resected with estradiol; area of GST-P-positive foci Further decrease. The area of GST-P-positive foci increased slightly after ovariectomy in female mice that had been treated with chemically-induced cancer alone; however, the area of positive foci was also increased in rats that received ovariectomy and testosterone. increase. Whether gonadectomy or not, male rats have higher GST-P expression than female rats. These results suggest that estrogen can be inhibited while androgen can promote the expression of GST-P in livers of chemically induced cancer rats. This result may be related to clinically more men than women susceptible to liver cancer.