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目的:测定不同产地栽培防风中升麻素苷,5-O-甲基维斯阿米醇苷及乙醇浸出物的含量,并进行质量比较。方法:高效液相色谱法,色谱柱为C_(18)(250mm×4.6mm,5μm),流动相:甲醇-水(4:6),检测波长254nm,流速0.8ml·min~(-1);乙醇热浸法提出有效成分。结果:产于河北安国的防风含升麻素苷、5-O-甲基维斯阿米醇苷和乙醇浸出物含量最高,分别为0.27%、0.26%和42.75%;产于甘肃兰州的含量最低,分别为0.12%、0.08%和22.15%。结论:河北安国、安徽亳州比较适宜防风栽培。
Objective: To determine the content of caffeine, 5-O-methylstilbenidoside and ethanol extracts in different habitats and to compare the quality. Method: High-performance liquid chromatography, column C_(18)(250mm×4.6mm, 5μm), mobile phase: methanol-water(4:6), detection wavelength 254nm, flow rate 0.8ml·min~(-1) Ethanol hot dip method proposes effective ingredients. RESULTS: The contents of the ascorbicin, 5-O-methylstilbenidoside and ethanol extracts produced in Hebei Anguo were the highest, which were 0.27%, 0.26% and 42.75%, respectively; the contents in Lanzhou, Gansu Province were as follows: The lowest was 0.12%, 0.08%, and 22.15%, respectively. Conclusion: Hebei Anguo and Chuzhou, Anhui Province are suitable for wind protection cultivation.