点滴复膜酵母Cyniclomyces guttulatu是一种定殖及生长于犬、兔、豚鼠、龙猫、大鼠和小鼠等动物胃肠道内的真菌.与大多数传统酵母相比,点滴复膜酵母具有耐酸(pH 1.5-4.5)、耐高温(38-42℃)的独特生长特性,可在体外快速增殖.腹泻动物粪便中可见大量点滴复膜酵母,尽管没有直接证据表明感染点滴复膜酵母会引起明显病状,但其被认为是多种动物的机会性致病菌.本研究通过全基因组测序和转录组测序明确点滴复膜酵母的基因结构和注释信息,获得点滴复膜酵母的系统性基因组和转录组数据.结果 显示基因组大小为29.71 Mb,包含11307个基因,转录组大小为17.67 Mb,GC含量分别为43.02％和43.09％;mRNA、CDS、外显子和内含子的平均长度分别为1476、1447、1374和540bp;点滴复膜酵母存在517个独特的基因家族,共包括1162个基因,该酵母的独特基因特点为后续研究奠定基础.比较分析结果表明,点滴复膜酵母的基因组大小和数量明显大于其他12种酵母,提示该酵母可能存在全基因组复制,这可能与其独特的胃肠道定殖、耐酸和耐高温生长特性密切相关.“,”Cyniclomyces guttulatus is a common inhabitant of the gastrointestinal tract of animals such as dogs,rabbits,guinea pigs,chinchillas,rats and mice.Compared with most common yeasts,C.guttulatus has unique adaptive characteristic to high concentration of acid (pH 1.5-4.5) and high temperature (38-42 ℃) and could proliferate rapidly in vitro.A large number of C.guttulatus was found in diarrhea animals.Although there was no direct evidence,the infection of C.guttulatus could lead to obvious diseases.Thus,this yeast may be considered as an opportunistic pathogen of a large number of animals.The objective of this study was to obtain a systematic genome and transcriptome data of C.guttulatus.The genes structure and annotation of C.guttulatus were obtained by the whole genome and transcriptome sequencing.It was confirmed that this yeast genome has a size of 29.71 Mb containing 11307 genes and the size of the transcriptome was 17.67 Mb;the GC content was 43.02％ and 43.09％,respectively.The average length of rnRNA,CDS,exons and introns were 1476,1447,1374 and 540 bp respectively.In total,517 unique gene families including 1162 genes were found in C.guttulatus.The results revealed that the genome size and the gene number of C.guttulatus are obviously larger than those of other 12 yeast species.A whole genome duplication even may have occurred in this species,which could explain its unique characteristics of colonization in the gastrointestinal tract and unique resistance to high concentration of acid and high temperature.