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目的:研究Ca~(2+)信号传导是否参与Hela细胞的信号传导过程以及小檗胺(Ber)对HeLa细胞内钙浓度([Ca~(2+)]_i)变化的影响。方法:Fluo 3-AM负载HeLa细胞,共聚焦法测定[Ca~(2+)]_i,结果以荧光强度(FI)表示。结果:(1)有外钙时,HeLa细胞静息FI为186±44,KCl、NE、Cal,及咖啡因均升高HeLa细胞的[Ca~(2+)]_i。(2)Ber处理后,静息FI无影响,但抑制KCl、NE和Cal引起的[Ca~(2+)]_i升高(P<0.01),FI变化的速率减慢,达峰值的时间延长。(3)无外钙时,咖啡因诱导的[Ca~(2+)]_i升高不被Ber抑制。(4)Ber的上述作用与Ver的作用相似。结论:HeLa细胞属于非兴奋性细胞,但部分生物学特征与兴奋性细胞相似,Ca~(2+)同样在其信息转导中发挥重要作用。
OBJECTIVE: To investigate whether Ca2 + signaling is involved in the signal transduction of Hela cells and the effect of berbamine on the intracellular calcium concentration ([Ca2 +] i) in HeLa cells. Methods: HeLa cells were loaded with Fluo 3-AM and [Ca 2+] i was measured by confocal method. The results were expressed as fluorescence intensity (FI). Results: (1) The resting FI of HeLa cells was 186 ± 44 with external calcium, and [Ca ~ (2 +)] _i of HeLa cells increased with KCl, NE, Cal and caffeine. (2) After treatment with Ber, resting FI had no effect, but inhibited the increase of [Ca2 +] i (P <0.01) caused by KCl, NE and Cal, and the rate of change of FI slowed down, reaching the peak time extend. (3) Caffeine-induced [Ca2 +] i increase was not inhibited by Ber without calcium supplementation. (4) The above effect of Ber is similar to that of Ver. CONCLUSION: HeLa cells belong to non-excitatory cells, but some of them are similar to excitatory cells in biological characteristics. Ca 2+ also play an important role in information transduction.