Altromycin B属pluramycin抗肿瘤抗生素类,包括Kidamycin,Hedamycin,pluramycin,neoplu-ramycin,DC-92B和Rubiflavin A。这类强力抗肿瘤药与DNA反应的方式尚不明确。本研究用凝胶电脉、磁共振及质谱分析法测定Altromycin B-DNA相加物的结构。高分辨凝胶电脉表明反应碱基是鸟嘌呤,而且N_7去氮杂鸟嘌呤代替鸟嘌呤进行的DNA链破裂实验结果证明与N_7有关。用超螺旋DNA的实验表明,Altromycin B和有关药物可以插 Altromycin B is a pluramycin antitumor antibiotic, including Kidamycin, Hedamycin, pluramycin, neoplu-ramycin, DC-92B, and Rubiflavin A. The way this powerful anti-cancer drug reacts with DNA is not yet clear. In this study, gel electrophoresis, magnetic resonance and mass spectrometry were used to determine the structure of Altromycin B-DNA adducts. High-resolution gel electrophoresis shows that the reaction base is guanine, and N7 deazaguanine instead of guanine DNA strand breaking test results show that N7-related. Experiments with supercoiled DNA showed that Altromycin B and related drugs could be inserted