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为探讨大鼠胃窦部胃泌素ｍＲＮＡ和生长抑素ｍＲＮＡ在Ｇ、Ｄ细胞的转录及其相关蛋白的表达，用免疫细胞化学和原位杂交技术，检测了大鼠胃窦部Ｇ细胞内的胃泌素和Ｄ细胞内的生长抑素以及它们相应的ｍＲＮＡ。结果显示，大鼠胃窦部的Ｇ、Ｄ细胞位于幽门腺基部，细胞分布不均，单个或多个在一起；胃泌素和生长抑素均匀地分布于胞质内，核内阴性；Ｇ／Ｄ细胞比值在１．３±０．３２～１．５５±０．７５之间，即Ｇ细胞多于Ｄ细胞。ｍＲＮＡ信号染色强度细胞间有差异，呈极性分布，多位于核周或核上胞质内。ｍＲＮＡ阳性细胞数在单位面积内少于Ｇ、Ｄ免疫组织化学显示阳性细胞数，原因可能是由于多聚甲醛的固定对ｍＲＮＡ的降解，降低了ｍＲＮＡ的活性，从而导致某些Ｇ、Ｄ细胞内ｍＲＮＡ不能被检出。该法具有安全、省时、定位准确等优点，适合一般实验室和临床诊断需要
In order to investigate the mRNA and protein expression of gastrin mRNA and somatostatin mRNA in G, D cells in gastric antrum of rats, immunocytochemistry and in situ hybridization Gastrin and somatostatin in D cells and their corresponding mRNAs. The results showed that the G and D cells located in the antrum of gastric antrum were located in the basement of pyloric gland. The cells were unevenly distributed and single or multiple together. Gastrin and somatostatin were evenly distributed in the cytoplasm and were negative in the nucleus. / D cell ratio between 1.3 ± 0.32 ~ 1.55 ± 0.75, that G cells more than D cells. mRNA signal staining intensity differences between cells were polar distribution, and more in the nuclear or nuclear cytoplasm. The number of mRNA positive cells in the unit area less than G, D immunohistochemistry showed that the number of positive cells may be due to paraformaldehyde fixed mRNA degradation, reducing mRNA activity, resulting in certain G, D cells mRNA can not be detected. The method is safe, time-saving, accurate positioning, etc., suitable for general laboratory and clinical diagnosis needs