作者介绍了一种改良的免疫印迹法,用收获的细胞培养物作为抗原,不需纯化病毒,用放射性碘标记的抗人IgG单克隆抗体(McAb)作为标准试剂即可完成。将感染HTLV-ⅢB株的H9或CEM细胞在36～37℃培养以增殖病毒,离心收集细胞,悬浮于含0.1M Tris-HCl 4%十二烷基硫酸钠(SDS)、4%2-巯基乙醇、20%蔗糖和0.001%溴酚蓝的溶解 The authors describe an improved immunoblot assay using harvested cell culture as antigen without virus purification and radioiodinated anti-human IgG monoclonal antibody (McAb) as a standard reagent. H9 or CEM cells infected with the HTLV-IIIB strain were cultured at 36-37C to proliferate the virus, and the cells were harvested by centrifugation and resuspended in 0.1 M Tris-HCl 4% sodium dodecyl sulfate (SDS), 4% 2-mercapto Ethanol, 20% sucrose and 0.001% bromophenol blue