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目的探讨咪达唑仑与在体小鼠肺泡液体清除率(AFC)之间的关系,以及β2肾上腺素受体激动剂特布他林对其作用的影响。方法应用酶标仪测定小牛血清白蛋白浓度的方法测定小鼠在体AFC。结果气管内注入0.1mmol/L咪达唑仑后,能显著降低小鼠AFC。与1mmol/L阿米洛利(特异性钠通道阻断剂)合用后抑制效应未见进一步增强,表明咪达唑仑能够抑制与上皮钠通道有关的阿米洛利敏感性AFC。β2肾上腺素受体激动剂特布他林能明显增加小鼠AFC,与咪达唑仑合用后,特布他林几乎完全逆转咪达唑仑对AFC的抑制作用。结论临床上对合并肺脏损害的患者应用咪达唑仑时应考虑其可能对肺脏液体清除作用的影响,必要时可以考虑应用β2肾上腺素受体激动剂特布他林进行治疗。
Objective To investigate the relationship between midazolam and alveolar fluid clearance (AFC) in mice and the effect of beta 2 adrenergic agonist terbutaline on it. Methods The concentration of bovine serum albumin was determined by microplate reader. Results Intratracheal instillation of 0.1 mmol / L midazolam significantly reduced AFC in mice. Combined with 1 mmol / L amiloride (specific sodium channel blocker) inhibition effect was not further enhanced, indicating that midazolam can inhibit epithelial sodium channel-related amiloride-sensitive AFC. Terbutaline, a beta 2 adrenergic receptor agonist, significantly increased AFC in mice and terbutaline reduced the inhibition of AFC by midazolam almost completely after combination with midazolam. Conclusions Clinically, the effect of midazolam on lung fluid clearance should be considered in patients with lung injury, and treatment with terbutaline, a β2 adrenergic receptor agonist, may be considered if necessary.