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本发明公开了一种基于纳米金化学发光快速检测三聚氰胺的方法,包括以下步骤:(1)移取纳米金溶液和含有三聚氰胺的溶液于一离心管中,充分混匀,作用5 min使得纳米金达到最佳的团聚态;(2)移取纳米金溶液和三聚氰胺溶液的混合溶液于化学发光池中,采用静态注射的方式注入鲁米诺–过氧化氢化学发光试剂,通过IFFL–D流动注射化学发光分析仪测定并记录其化学发光强度,根据测定结果进行判定。本发明检出限为8.6×10–14 g/m L,达到目前固相萃取–化学发光分析法的灵敏度,且较之简单方便;分析测试时间缩短为10 min以内;整个操作实验条件较温和,有利于自动化的分析操作。
The invention discloses a method for rapidly detecting melamine based on nanogold chemiluminescence, which comprises the following steps: (1) taking a gold nanometer solution and a solution containing melamine in a centrifuge tube and thoroughly mixing for 5 min so that the nanometer gold (2) Pipette a mixed solution of nano-gold solution and melamine solution in a chemiluminescence cell, inject luminol-hydrogen peroxide chemiluminescence reagent by static injection, and inject the solution by IFFL-D flow injection Chemiluminescence analyzer measured and recorded its chemiluminescence intensity, according to the determination of the results of the decision. The detection limit of the present invention is 8.6 × 10-14 g / m L, which is up to the current sensitivity of the solid-phase extraction-chemiluminescence analysis method, and is simple and convenient; the analysis and test time is shortened to 10 minutes or less; the overall operating experimental condition is relatively mild , Is conducive to automated analysis operations.