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目的 研究慢性应激对老化过程的影响。方法 采用悬尾方式每天予以小鼠应激刺激,持续14d,每天适当延长悬吊时间(记下小鼠体温下降情况决定所延长时间,水温维持于23±2℃)。悬吊时,每天对小鼠进行性功能监测,记录10min内跨骑、交配次数。最后一次悬尾后12h,处死小鼠,取其全脑。检测脑组织老化相关酶-单胺氧化酶(MAO-B),超氧化物歧化酶(SOD)活性,及脂褐素沉积情况。结果 悬尾6d时,小鼠跨骑与交配次数与对照组相比有显著差别(P<0.05),小鼠性功能明显衰退。悬尾12d后,脑组织MAO-B活性显著升高(P<0.05),SOD活性显著下降(P<0.01),脂褐素沉积增加(P<0.05)。结论 慢性应激易化老化进程,使小鼠呈现类老化变化。
Objective To study the effect of chronic stress on aging process. Methods Mice were challenged with tail suspension every day for 14 days. The suspension time was prolonged every day. The temperature of mice was recorded and the temperature was maintained at 23 ± 2 ℃. Suspension, the mice daily sexual function monitoring, recording cross riding within 10min, the number of mating. After the last tail-suspended 12h, mice were sacrificed and the whole brain was taken. The activities of MAO-B, SOD and the lipofuscin deposition in brain tissue were detected. Results 6 d after tail suspension, the number of mice riding and mating compared with the control group were significantly different (P <0.05), the mice showed a significant decline in sexual function. After 12 days, the activity of MAO-B in brain tissue was significantly increased (P <0.05), SOD activity was significantly decreased (P <0.01), lipofuscin deposition was increased (P <0.05). Conclusion Chronic stress can accelerate the process of aging and make the mice show the similar aging changes.