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目的探讨芹菜素对肺癌A549细胞迁移和侵袭能力的影响和机制。方法采用0、5、10、20、40、80、160μmol/L浓度的芹菜素培养液与A549细胞共培养,利用CCK8法检测A549细胞的生存率,Transwell迁移和侵袭实验检测芹菜素对A549细胞迁移和侵袭能力的影响,酶联免疫吸附实验(ELISA)、蛋白质印迹法分析芹菜素对细胞基质金属蛋白酶(MMP-2、MMP-9)和血管内皮生长因子(VEGF)活性和蛋白表达的改变。结果 CCK8显示40~160μmol/L的芹菜素可抑制A549细胞增殖,并呈浓度依赖性(P<0.05);与对照组比较,经芹菜素处理后A549细胞迁移和侵袭能力降低,相对应的MMP-2、VEGF蛋白表达和分泌减少(P<0.05);但对于MMP-9,芹菜素不能抑制其蛋白的表达和分泌。结论芹菜素可有效抑制肺癌A549细胞迁移和侵袭能力,其机制可能与芹菜素降低细胞MMP-2、VEGF的蛋白表达和分泌相关。
Objective To investigate the effect and mechanism of apigenin on lung cancer A549 cell migration and invasion. Methods A549 cells were co-cultured with apigenin at 0, 5, 10, 20, 40, 80 and 160μmol / L concentrations. A549 cells were detected by CCK8 assay. Transwell migration and invasion assays were used to determine the effect of apigenin on A549 cells Migration and invasive ability, the changes of cell matrix metalloproteinase (MMP-2, MMP-9) and vascular endothelial growth factor (VEGF) activity and protein expression were analyzed by enzyme-linked immunosorbent assay (ELISA) and Western blotting . Results CCK8 showed that apigenin at a concentration of 40 ~ 160μmol / L inhibited the proliferation of A549 cells in a concentration-dependent manner (P <0.05). Compared with the control group, the ability of migration and invasion of A549 cells decreased after treatment with apigenin, -2, VEGF protein expression and secretion decreased (P <0.05); but for MMP-9, apigenin can not inhibit the protein expression and secretion. Conclusions Apigenin can effectively inhibit the migration and invasion ability of lung cancer A549 cells. The mechanism may be related to the decrease of the protein expression and secretion of MMP-2 and VEGF by apigenin.