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以两个转Bt基因抗虫玉米品系G03-2396、G03-2739和对照玉米品种苏玉16为材料,采用室内生物测定法研究它们对亚洲玉米螟的抗性,并采用酶联免疫技术(ELISA)检测这两个转基因玉米品系不同组织中Bt毒蛋白的表达量及亚洲玉米螟3龄与5龄幼虫取食转基因玉米后体内和粪便中的Bt毒蛋白含量.结果表明:转Bt基因抗虫玉米心叶对玉米螟幼虫的毒性较强,初孵幼虫取食6 d后的存活率不到3%,3龄幼虫取食6 d后的存活率小于70%,抗虫玉米雌穗的毒性小于心叶.两个转Bt基因玉米心叶和雌穗中均表达了一定量的Bt毒蛋白,但心叶中的毒蛋白含量高于雌穗;Bt毒蛋白表达量依次为G03-2739心叶(39.6μg.g-1FM)>G03-2396心叶(26.1μg.g-1FM)>G03-2396雌穗(17.0μg.g-1FM)>G03-2739雌穗(14.6μg.g-1FM).取食转基因玉米心叶或雌穗后,3龄幼虫体内的Bt毒蛋白含量显著高于5龄幼虫;同龄幼虫取食心叶后其体内及粪便中Bt毒蛋白含量均显著高于取食雌穗的个体.其中,取食G03-2739心叶的5龄幼虫粪便中的Bt毒蛋白含量最高,达10.4μg.g-1FM;取食其雌穗的3龄幼虫粪便中的Bt毒蛋白含量最低,仅2.7μg.g-1FM.
Two transgenic Bt corn lines G03-2396, G03-2739 and control corn variety Suyu 16 were used as materials to study their resistance to Asian corn borer by indoor bioassay and ELISA ) To detect Bt toxin protein expression in different tissues of the two transgenic maize strains and Bt toxin content in vivo and in feces of 3rd and 5th instar larvae of Asian corn borer.The results showed that Bt toxin The corn leaf had stronger toxicity to corn borer larvae. The survival rate of newly hatched larvae was less than 3% after 6 days of feeding. The survival rate of 3rd instar larvae after 6 days of feeding was less than 70% Less than the heart.Both Bt toxin and Bt toxin were expressed in the leaves and ears of both transgenic Bt corn, but the content of toxic protein in the heart leaves was higher than that of the female ear. The expression levels of Bt toxin were G03-2739 Leaves (39.6 μg.g-1FM)> G03-2396 heart leaves (26.1 μg.g-1FM)> G03-2396 ears (17.0 μg.g-1FM)> G03-2739 ears (14.6 μg.g-1FM) ) .While feeding on the leaves or ears of transgenic corn, Bt toxic protein content of 3rd instar larvae was significantly higher than that of 5th instar larvae. Bt virulent eggs Were significantly higher than that of the individuals fed on the female ear, in which the 5th instar larvae of G03-2739 fed the stool with the highest Bt protein content of 10.4μg.g-1FM, and the 3rd instar The larval excrement of Bt toxic protein content of the lowest, only 2.7μg.g-1FM.