论文部分内容阅读
目的:研究疏经防痛胶囊的质量控制方法。方法:采用TLC对疏经防痛胶囊中丹参、当归、乳香及没药进行定性鉴别;采用HPLC方法,以乙腈-0.1%磷酸溶液为流动相,进行梯度洗脱,在检测波长230 nm处同时测定芍药苷、柚皮苷、新橙皮苷及丹酚酸B共4种成分含量。结果:TLC鉴别分离度好,专属性强。芍药苷在25.30~506.00 mg·L~(~(-1)),柚皮苷在12.68~253.60 mg·L~(~(-1)),新橙皮苷在15.24~304.80 mg·L~(~(-1)),丹酚酸B在27.72~554.30 mg·L~(~(-1))分别具有良好的线性关系,相关系数分别为1.000 0,0.999 9,0.999 9,0.999 9,平均加样回收率分别为100.69%(RSD 0.9%),100.08%(RSD 1.6%),100.85%(RSD 1.2%)和100.42%(RSD 2.0%)。结论:该质量控制方法简便、准确、重复性好,可有效的控制疏经防痛胶囊质量。
Objective: To study the quality control method of Shujing anti-pain capsule. Methods: TLC was used to identify the Danshen, Angelica, frankincense and myrrh in Danshen anti-pain capsule. The HPLC method was used with gradient elution with acetonitrile-0.1% phosphoric acid solution as the mobile phase at the detection wavelength of 230 nm The contents of paeoniflorin, naringin, neohesperidin and salvianolic acid B were determined. Results: TLC identification of good resolution, specificity. The concentrations of paeoniflorin in the range of 25.30 ~ 506.00 mg · L ~ (-1), naringin in 12.68 ~ 253.60 mg · L ~ (-1)) and neohesperidin in the range of 15.24 ~ 304.80 mg · L ~ (-1) ~ (-1)), respectively. There was a good linear relationship between salvianolic acid B (27.72 ~ 554.30 mg · L ~ (-1)) and the correlation coefficients were 1.000 0,0.999 9,0.999 9,0.999 9, The recoveries were 100.69% (RSD 0.9%), 100.08% (RSD 1.6%), 100.85% (RSD 1.2%) and 100.42% (RSD 2.0%), respectively. Conclusion: The quality control method is simple, accurate, reproducible, and can effectively control the quality of Shuzhu Fangtong Capsule.