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目的 探讨胃良恶性病变组织中端粒酶表达情况及其与幽门螺杆菌 (H .pylori)感染的关系。 方法 采用端粒酶TRAP ELISA方法检测 1 9例慢性浅表性胃炎 (CSG)、2 9例胃癌 (GC)、69例胃良性溃疡(GU)组织端粒酶活性 ,并分析三组H .pylori感染与端粒酶表达的关系。 结果 CSG、GU、GC三组端粒酶阳性表达率分别为 0 %、36 2 % (2 5/ 69)和 79 3 % (2 3/ 2 9)。其中GU组中无伴肠上皮化生及异型增生的 2 5例组织中端粒酶阳性率为 8% (2 / 2 5) ;GU伴肠上皮化生的为 40 % (6/ 1 5) ;GU伴异型增生的为 58 6 % (1 7/ 2 9)。CSG组与GC组及GU组比较、GU组与GC组比较 χ2 =2 4 6～ 9 6 ,P <0 0 1 ;GU无伴肠上皮化生及异型增生的与GU伴有肠上皮化生及异型增生的比较 χ2 =1 5 2 ,P <0 0 1 ;GU伴肠上皮化生的与GU伴异型增生的比较 χ2 =1 1 ,P >0 0 5。CSG、GU和GC组H .pylori感染率分别为 31 6 %、65 3 %和 44 8% ,CSG组与GC组比较、GU组与GC组比较差异无显著意义 (P >0 0 5) ;CSG组与GU组比较差异有显著意义 (χ2 =6 91 ,P<0 0 1 ) ,各组中H .pylori阳性组端粒酶阳性表达率与H .pylori阴性组端粒酶阳性表达率间比较差异均无显著意义 (P均 >0 0 5)。结论 端粒酶激活是胃癌发生的早期事件 ,但可能与H .pylori?
Objective To investigate the expression of telomerase in benign and malignant gastric lesions and its relationship with Helicobacter pylori infection. Methods Telomerase activity was detected by TRAP ELISA in 17 cases of chronic superficial gastritis (CSG), 29 cases of gastric cancer (GC) and 69 cases of benign gastric ulcer (GU). The levels of telomerase activity in three groups of H. pylori Relationship between infection and telomerase expression. Results The positive rates of telomerase in CSG, GU and GC groups were 0%, 36 2% (25/69) and 79 3% (2 3/2 9), respectively. The positive rate of telomerase was 25% (2/25) in 25 cases of GU without intestinal metaplasia and dysplasia; the rate of GU with intestinal metaplasia was 40% (6/15) ; GU with dysplasia was 58 6% (17/29). Compared with GC group and GU group, GUG group and GC group χ2 = 246 ~ 96, P <0 01; GU associated with intestinal metaplasia and dysplasia GU associated with intestinal metaplasia And dysplasia compared χ2 = 1 5 2, P <0 0 1; GU associated with intestinal metaplasia and GU with dysplasia compared χ2 = 1 1, P> 0 0 5. The infection rates of H.pylori in CSG, GU and GC groups were 31.6%, 65.3% and 44.8% respectively. There was no significant difference between GC group and GCG group (P> 0.05). There was significant difference between CSG group and GU group (χ2 = 6 91, P <0.01). The positive rates of telomerase between H.pylori positive group and telomerase positive rate between H.pylori negative group There was no significant difference (P> 0.05). Conclusion Telomerase activation is an early event in gastric cancer, but may be related to H. pylori?