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目的探讨聚腺苷酸二磷酸核糖聚合酶-1[Poly(ADP-ribose)polymerase-1,PARP-1]抑制剂PJ34对卵巢癌耐药细胞株C13*细胞生长活性及耐药性的影响。方法应用四甲基偶氮唑蓝(MTT)及流式细胞术检测PJ34对C13*细胞顺铂敏感性、凋亡和周期的影响;免疫荧光及蛋白印迹法(Western blot)检测PJ34处理前后PARP-1的表达情况。结果 C13*细胞增殖抑制率及对顺铂的敏感性随顺铂及PJ34浓度的增加而增强(P<0.05);细胞凋亡率随PJ34浓度的增加而增高(P<0.05);周期为PJ34浓度越高,G0/G1期细胞越多,S期细胞越少;PJ34可下调细胞中PARP-1的表达,顺铂与PJ34合用后,PARP-1蛋白表达量明显降低(P<0.01)。结论 PJ34能显著下调C13*细胞内PARP-1表达,抑制细胞增殖,促进细胞凋亡,增加细胞对顺铂的敏感性,有效逆转C13*细胞对顺铂的耐药性。
Objective To investigate the effect of poly (ADP-ribose) polymerase-1 (PARP-1) inhibitor PARJ3 on the growth and drug resistance of ovarian cancer cell line C13 *. Methods MTT and flow cytometry were used to detect the effect of PJ34 on Cisplatin sensitivity, apoptosis and cycle in C13 * cells. Immunofluorescence and Western blot were used to detect the expression of PARP -1 expression. Results The inhibitory rate of C13 * cell proliferation and the sensitivity to cisplatin were increased with the increase of cisplatin and PJ34 concentration (P <0.05). The apoptosis rate of C13 * cells increased with the increase of PJ34 concentration (P <0.05) PJ34 could down-regulate the expression of PARP-1 in the G0 / G1 phase cells, and decreased the expression of PARP-1 protein (P <0.01). Conclusion PJ34 can significantly down-regulate the expression of PARP-1 in C13 * cells, inhibit cell proliferation, promote apoptosis and increase cell sensitivity to cisplatin, effectively reversing the drug resistance of C13 * cells to cisplatin.