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在不施肥和施用猪粪两种情况下,采用培养试验研究了不同浓度土霉素污染(0、0.1、1、10、100和1000 mg.kg-1)对土壤细菌丰度、酶活性和NO3--N浓度等的影响.试验培养温度为25℃,培养时间为30 d,取样分析时间分别为1、4和30 d.结果表明:在不施肥条件下,土霉素污染对土壤细菌数量及微生物活性的影响较小,土壤S1、S2和S3细菌数量、呼吸强度、酶活性和NO3--N浓度下降10%时土霉素的剂量(EC10)分别为36~1000 mg.kg-1、20~1000 mg.kg-1和4~1000 mg.kg-1;而在施用猪粪的情况下,对应的数值分别为2~656 mg.kg-1、2~81 mg.kg-1和1~42 mg.kg-1.添加土霉素对土壤细菌及酶活性的影响随土壤肥力的提高而增大,且其对土壤细菌数量和呼吸强度的影响大于对酶活性和NO3--N浓度的影响.土霉素污染对土壤微生物数量和活性的影响随时间变化而变化,一般在培养4 d时的影响最为明显.土霉素对土壤微生物的影响总体上表现为抑制作用.
Under the condition of no fertilizer application and pig manure application, the culture experiments were conducted to study the effects of different concentrations of oxytetracycline (0, 0.1, 1, 10, 100 and 1000 mg.kg-1) on soil bacterial abundance, NO3 - N concentration, etc. The incubation temperature was 25 ℃, the incubation time was 30 d, and the sampling and analysis times were 1, 4 and 30 d, respectively.The results showed that under the condition of no fertilization, Quantity and microbial activity of the soil, and the dose of oxytetracycline (EC10) was 36 ~ 1000 mg.kg-1 for soil S1, S2 and S3 respectively when the bacterial population, respiration rate, enzyme activity and NO3- 1, 20-1000 mg.kg-1, and 4-1000 mg.kg-1, respectively, whereas in the case of pig manure, the corresponding values were 2-656 mg.kg-1 and 81-81 mg.kg- 1 and 1 ~ 42 mg.kg-1. The effects of oxytetracycline on soil bacteria and enzyme activities increased with the increase of soil fertility, and its effect on soil bacterial population and respiration intensity was greater than that of soil enzyme activities and NO3- -N concentration.Effect of oxytetracycline on soil microbial quantity and activity changes with time, usually the most obvious impact on the 4 d culture.The impact of oxytetracycline on soil microorganisms total Inhibitory effect on performance.