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本实验通过对有氧运动训练 (无负重游泳 6 0分钟 /天 ,7天 )大鼠游泳运动后的骨骼肌、心肌线粒体质子跨膜转运能力及核糖核苷二磷酸还原酶活性的测定 ,发现有氧运动训练的大鼠在定量运动负荷后 (6 0分钟无负重游泳 ) ,骨骼肌线粒体的质子跨膜转运能力显著提高 (P <0 0 5 ) ,以及线粒体的核糖核苷二磷酸还原酶活性明显高于对照组 (未经训练之大鼠 ,P <0 0 0 1)。而心肌线粒体的以上两项指标变化不甚明显。结果显示 ,骨骼肌线粒体对有氧运动训练的适应过程与其质子跨膜转运能力的提高及核糖核苷二磷酸还原酶活性增加有关。提示骨骼肌线粒体在慢性高氧化磷酸化状态刺激下 ,可能同时导致DNA生物合成的增加 ,即线粒体基因组对其功能变化产生应答反应。心肌线粒体的运动适应过程与骨骼肌线粒体不尽相同。
In this experiment, the aerobic exercise training (no-load swimming 6 0 minutes / day, 7 days) after swimming swimming rat skeletal muscle, myocardial mitochondrial proton transmembrane transport capacity and determination of ribonucleoside diphosphate reductase activity and found that After aerobic exercise training, the proton transmembrane transport ability of skeletal muscle mitochondria was significantly increased (P <0.05) after quantitative exercise load (without weight-bearing swimming for 60 minutes), and mitochondrial ribonucleoside diphosphate reductase Activity was significantly higher than the control group (untrained rats, P <0 0 0 1). Myocardial mitochondrial changes in the above two indicators is not obvious. The results showed that the adaptive process of skeletal muscle mitochondria to aerobic exercise training was related to the enhancement of proton transmembrane transport ability and the increase of ribonucleoside diphosphate reductase activity. This suggests that stimulation of skeletal muscle mitochondria may lead to the increase of DNA biosynthesis at the same time under the condition of chronic hyperphosphorylation, that is, the mitochondrial genome responds to its functional changes. Myocardial mitochondrial motor adaptation process and skeletal muscle mitochondria vary.