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目的:了解胃癌细胞凋亡与凋亡调控基因Bcl2、P53蛋白表达的关系,并探讨胃癌细胞凋亡与组织学分型的关系。方法:采用原位末端标记法(TUNEL)检测细胞凋亡,采用ABC免疫组化法检测Bcl2和P53蛋白的表达。结果:从高分化、中分化到低—未分化腺癌的细胞凋亡指数逐步下降,而Bcl2和P53蛋白表达无显著差异。在Lauren分型中,肠型胃癌与弥漫型胃癌比较,细胞凋亡指数高、Bcl2蛋白低表达及P53蛋白高表达。结论:胃癌的细胞分化程度与细胞凋亡指数密切相关,而与Bcl2和P53表达无关。胃癌的Lauren分型与细胞凋亡指数、Bcl2蛋白表达及P53蛋白表达密切相关。
Objective: To investigate the relationship between the apoptosis of gastric cancer cells and the expression of apoptosis-regulating genes Bcl-2 and P53, and to explore the relationship between the apoptosis of gastric cancer cells and histological types. Methods: Apoptosis was detected by TUNEL. ABC immunohistochemistry was used to detect the expression of Bcl 2 and P53 protein. RESULTS: The apoptotic index decreased gradually from well-differentiated to moderately-differentiated adenocarcinoma, but there was no significant difference in the expression of Bcl-2 and P53 protein. In the Lauren classification, compared with diffuse type gastric cancer, intestinal type gastric cancer has high apoptosis index, low expression of Bcl 2 protein and high expression of P53 protein. Conclusion: The degree of cell differentiation in gastric cancer is closely related to the apoptosis index, but not related to the expression of Bcl 2 and P53. The Lauren classification of gastric cancer is closely related to apoptosis index, Bcl 2 protein expression and P53 protein expression.