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探讨金柴抗病毒胶囊对甲型H1N1流感病毒FM/1/47株感染小鼠肺炎模型的治疗作用机制。滴鼻感染正常小鼠,建立流感病毒肺炎模型并进行治疗性给药。采用实时荧光定量PCR法、Western blot在流感病毒感染后第1、3、5、7天检测小鼠肺组织中病毒载量、干扰素诱导跨膜蛋白的动态变化。实验结果显示,金柴抗病毒胶囊可降低肺组织病毒载量,其中大、中、小3个剂量组在病毒感染正常小鼠后各检测时间点均能明显降低肺组织中病毒载量的表达(P<0.05,P<0.01)。金柴抗病毒胶囊可上调干扰素诱导跨膜蛋白相对表达量。与模型对照组比较,其中大剂量在各个时间点均明显高表达(P<0.05,P<0.01),中剂量在第3、5天具有明显高表达(P<0.05);小剂量组在第3天表现显著高表达(P<0.05)。实验结果表明:金柴抗病毒胶囊可能通过上调流感病毒感染小鼠肺组织内干扰素诱导跨膜蛋白表达量来发挥抗流感病毒的作用。
To investigate the therapeutic effect of Jinchai Antiviral Capsule on the pneumonia model induced by influenza A (H1N1) virus FM1 / 47 in mice. Nasal infection in normal mice, the establishment of influenza virus pneumonia model and for therapeutic administration. The real-time fluorescent quantitative PCR method was used to detect the viral load in the lung tissue of mice on the 1st, 3rd, 5th and 7th day after infection with the influenza virus. The dynamic changes of IFN-induced transmembrane proteins were detected. The results showed that the injection of Jinchai antivirus could reduce the viral load of lung tissue. Among the three groups, the three groups of large, medium and small doses could significantly reduce the expression of viral load in lung tissue at each detection time point after the virus was infected in normal mice (P <0.05, P <0.01). Jinchai antivirus capsule can up-regulate the relative expression of the trans-membrane protein induced by interferon. Compared with the model control group, the high dose group was significantly higher than that of the model control group at all time points (P <0.05, P <0.01), and middle dose was significantly higher on the 3rd and 5th day (P <0.05) 3 days significantly higher expression (P <0.05). Experimental results show that Jinchai Antiviral Capsule may play an anti-influenza virus effect by up-regulating interferon-inducible transmembrane protein expression in the lung tissue of mice infected with influenza virus.