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目的探讨普通荧光定量PCR技术与内标法荧光定量PCR技术在乙型肝炎病毒(HBV)检测中的应用评价。方法采集郑州大学第一附属医院2013年6月-2014年6月HBV患者的血浆112份,分别用上述方法进行检测。配对卡方检验比较阳性检出率;观察普通法检测<500 IU/ml者的内标法结果;应用配对资料秩和检验正态近似法分析阳性结果差异。结果 112例标本中,内标法阳性率为77%(86/112);普通法阳性率为33%(37/112),两者差异具有统计学意义(P>0.05);37例普通法>500 IU/ml的标本,内标法结果均为阳性;75例普通法<500 IU/ml的标本,内标法结果26例为阴性,49例为阳性,其中23例<20 IU/ml,21例为20 IU/ml~300 IU/ml;2种方法检测HBV病毒阳性标本共37例,病毒载量统计分析差异无统计学意义(Z=0.717,P>0.05)。结论 2种方法均可用于活动期或高复制阶段患者检测对低复制阶段患者检测、治疗效果评估及预后监测,使用内标法更为合理。
Objective To evaluate the application of fluorescence quantitative PCR and internal standard fluorescence quantitative PCR in the detection of hepatitis B virus (HBV). Methods A total of 112 plasma samples of HBV patients from June 2013 to June 2014 in the First Affiliated Hospital of Zhengzhou University were collected and tested by the above methods. Paired chi-square test to compare the positive detection rate; observe common method test <500 IU / ml internal standard method; paired data rank sum test normal approximation analysis positive result difference. Results Among the 112 specimens, the positive rate of internal standard was 77% (86/112); the positive rate of common method was 33% (37/112), the difference was statistically significant (P> 0.05); 37 common methods > 500 IU / ml, the internal standard results were positive; 75 cases of common law <500 IU / ml specimens, the internal standard results of 26 cases were negative, 49 cases were positive, of which 23 cases <20 IU / ml , And 21 cases were 20 IU / ml to 300 IU / ml. There were 37 cases of HBV positive samples detected by two methods. There was no significant difference in viral load between two groups (Z = 0.717, P> 0.05). Conclusions Both methods can be used to detect patients in low replication stage, assess the therapeutic effect and monitor the prognosis of patients during active phase or high replication phase. It is more reasonable to use internal standard method.